Construct: ORF TRCN0000478421

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012145.2_s317c1
Derived from:
ccsbBroadEn_02851
DNA Barcode:
GAGAAACCTTTGGCACTATACGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SUN2 (25777)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478421
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25777 SUN2 Sad1 and UNC84 domain conta... NM_001199580.2 100% 100%
2 human 25777 SUN2 Sad1 and UNC84 domain conta... NM_015374.3 100% 100%
3 human 25777 SUN2 Sad1 and UNC84 domain conta... XM_024452205.1 100% 100%
4 human 25777 SUN2 Sad1 and UNC84 domain conta... NM_001199579.2 97.1% 97.1% 424_486del
5 human 25777 SUN2 Sad1 and UNC84 domain conta... XM_024452204.1 95.3% 95.3% 1_105del
6 human 25777 SUN2 Sad1 and UNC84 domain conta... XM_017028748.1 95.1% 94.8% (many diffs)
7 human 25777 SUN2 Sad1 and UNC84 domain conta... XM_011530104.2 93.5% 93.5% 284_285ins138
8 human 25777 SUN2 Sad1 and UNC84 domain conta... XM_024452206.1 93.5% 93.5% 284_285ins138
9 human 25777 SUN2 Sad1 and UNC84 domain conta... XM_011530105.2 89.2% 89.2% 1_105del;389_390ins138
10 human 25777 SUN2 Sad1 and UNC84 domain conta... XM_024452203.1 88.5% 88.5% 1_105del;391_564del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2220
ORF length:
2151
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtcccgaaga agccagcgcc tcacgcgcta ctcccagggt gacgatgacg
121gcagcagcag cagcggaggg agctcggtgg ctgggagtca gagcaccctg tttaaagaca
181gtcctctcag gaccttgaag aggaaatcca gcaacatgaa gcgcctgtcc ccagcgccac
241agctgggccc gtcctctgat gcacacacct cctactacag tgagtcgctg gtccacgagt
301cctggttccc acccaggagc tccctggagg aactgcatgg tgacgccaac tggggtgagg
361acctgcgggt gcggaggagg agaggcacgg gtggctcaga gagcagcagg gccagcgggc
421ttgtggggcg caaggccacc gaggacttcc tgggctcttc ctcgggctac tcctctgagg
481acgactacgt gggctactcg gatgtggacc agcagagttc cagctcgcgg ctccgaagcg
541ccgtctcacg ggcgggctcc ttactctgga tggtggccac ttcgccaggc cggctcttca
601gacttctcta ctggtgggct ggcaccacct ggtaccgcct gaccacagct gcctccctcc
661ttgacgtctt cgttttaacc aggcgcttct cgtccctgaa gacgttcctc tggttcctgc
721tgccgctgct cttgctgacg tgcctgacgt atggtgcttg gtatttctac ccctatgggc
781tgcagacatt ccaccctgct ttggtttcct ggtgggcagc gaaggacagc aggaggccgg
841atgagggctg ggaagccaga gactcatcgc cacatttcca ggctgagcag cgtgttatgt
901cccgggtaca ctctctggag cggcgtctgg aagctcttgc tgctgaattt tcctccaact
961ggcagaagga ggccatgcgg ctggaacgtc tggagctgcg gcaaggggct cctggccagg
1021gaggtggtgg tggcctgagc cacgaggaca ccctggcgct gctggagggg ctagtgagcc
1081gccgtgaagc tgccctgaag gaggatttcc gcagggaaac tgctgctcgc atccaggaag
1141aactgtctgc cctgagagca gagcatcagc aagactcaga agacctcttc aagaagatcg
1201tccgggcctc ccaggagtcc gaggctcgca tccagcagct gaagtcagag tggcaaagca
1261tgacccagga gtccttccag gagagctctg tgaaggagct gaggcggctg gaggaccagc
1321tggccggcct gcagcaggag ctggcggctc tggcactgaa gcagagctcg gtggcggaag
1381aagtgggcct gctgccccag cagatccagg ccgtgcggga cgacgtggaa tctcagttcc
1441cggcctggat cagtcagttc cttgcccgag gtggaggggg ccgcgtgggg ctccttcaga
1501gagaggagat gcaagctcag ctgcgagagc tggagagcaa gatcctcacc catgtggcag
1561agatgcaggg caagtcggcc agggaagccg cggcctccct gagcctgacg ctgcagaaag
1621aaggtgtgat tggagtgaca gaggagcagg tgcaccacat cgtgaagcag gccctgcagc
1681gctacagtga ggaccgcatc gggctggcag actacgccct ggagtcagga ggggccagcg
1741tcatcagcac ccgatgttct gagacctacg agaccaagac ggccctcctc agcctcttcg
1801gcatccccct gtggtaccac tcccagtcac cccgagtcat cctccagcca gatgtgcacc
1861caggcaactg ctgggccttc caggggccac aaggcttcgc cgtggTCCGC CTCTCTGCCC
1921GCATCCGCCC CACAGCCGTT ACCTTAGAGC ATGTGCCCAA GGCCTTGTCA CCCAACAGCA
1981CTATCTCCAG TGCCCCCAAG GACTTCGCCA TCTTTGGGTT TGACGAAGAC CTGCAGCAGG
2041AGGGGACACT CCTTGGCAAG TTCACTTACG ATCAGGACGG CGAGCCTATT CAGACGTTTC
2101ACTTTCAGGC CCCTACGATG GCCACGTACC AGGTGGTGGA GCTGCGGATC CTGACTAACT
2161GGGGCCACCC CGAGTACACC TGCATCTACC GCTTCAGAGT GCATGGGGAG CCCGCCCACT
2221TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
2281GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2341TTTATATATC TTGTGGAAAG GACGAGAGAA ACCTTTGGCA CTATACGTCA CGCGTTAAGT
2401Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)