Construct: ORF TRCN0000478421
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012145.2_s317c1
- Derived from:
- ccsbBroadEn_02851
- DNA Barcode:
- GAGAAACCTTTGGCACTATACGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUN2 (25777)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478421
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | NM_001199580.2 | 100% | 100% | |
| 2 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | NM_015374.3 | 100% | 100% | |
| 3 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | XM_024452205.1 | 100% | 100% | |
| 4 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | NM_001199579.2 | 97.1% | 97.1% | 424_486del |
| 5 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | XM_024452204.1 | 95.3% | 95.3% | 1_105del |
| 6 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | XM_017028748.1 | 95.1% | 94.8% | (many diffs) |
| 7 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | XM_011530104.2 | 93.5% | 93.5% | 284_285ins138 |
| 8 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | XM_024452206.1 | 93.5% | 93.5% | 284_285ins138 |
| 9 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | XM_011530105.2 | 89.2% | 89.2% | 1_105del;389_390ins138 |
| 10 | human | 25777 | SUN2 | Sad1 and UNC84 domain conta... | XM_024452203.1 | 88.5% | 88.5% | 1_105del;391_564del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2220
- ORF length:
- 2151
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcccgaaga agccagcgcc tcacgcgcta ctcccagggt gacgatgacg 121 gcagcagcag cagcggaggg agctcggtgg ctgggagtca gagcaccctg tttaaagaca 181 gtcctctcag gaccttgaag aggaaatcca gcaacatgaa gcgcctgtcc ccagcgccac 241 agctgggccc gtcctctgat gcacacacct cctactacag tgagtcgctg gtccacgagt 301 cctggttccc acccaggagc tccctggagg aactgcatgg tgacgccaac tggggtgagg 361 acctgcgggt gcggaggagg agaggcacgg gtggctcaga gagcagcagg gccagcgggc 421 ttgtggggcg caaggccacc gaggacttcc tgggctcttc ctcgggctac tcctctgagg 481 acgactacgt gggctactcg gatgtggacc agcagagttc cagctcgcgg ctccgaagcg 541 ccgtctcacg ggcgggctcc ttactctgga tggtggccac ttcgccaggc cggctcttca 601 gacttctcta ctggtgggct ggcaccacct ggtaccgcct gaccacagct gcctccctcc 661 ttgacgtctt cgttttaacc aggcgcttct cgtccctgaa gacgttcctc tggttcctgc 721 tgccgctgct cttgctgacg tgcctgacgt atggtgcttg gtatttctac ccctatgggc 781 tgcagacatt ccaccctgct ttggtttcct ggtgggcagc gaaggacagc aggaggccgg 841 atgagggctg ggaagccaga gactcatcgc cacatttcca ggctgagcag cgtgttatgt 901 cccgggtaca ctctctggag cggcgtctgg aagctcttgc tgctgaattt tcctccaact 961 ggcagaagga ggccatgcgg ctggaacgtc tggagctgcg gcaaggggct cctggccagg 1021 gaggtggtgg tggcctgagc cacgaggaca ccctggcgct gctggagggg ctagtgagcc 1081 gccgtgaagc tgccctgaag gaggatttcc gcagggaaac tgctgctcgc atccaggaag 1141 aactgtctgc cctgagagca gagcatcagc aagactcaga agacctcttc aagaagatcg 1201 tccgggcctc ccaggagtcc gaggctcgca tccagcagct gaagtcagag tggcaaagca 1261 tgacccagga gtccttccag gagagctctg tgaaggagct gaggcggctg gaggaccagc 1321 tggccggcct gcagcaggag ctggcggctc tggcactgaa gcagagctcg gtggcggaag 1381 aagtgggcct gctgccccag cagatccagg ccgtgcggga cgacgtggaa tctcagttcc 1441 cggcctggat cagtcagttc cttgcccgag gtggaggggg ccgcgtgggg ctccttcaga 1501 gagaggagat gcaagctcag ctgcgagagc tggagagcaa gatcctcacc catgtggcag 1561 agatgcaggg caagtcggcc agggaagccg cggcctccct gagcctgacg ctgcagaaag 1621 aaggtgtgat tggagtgaca gaggagcagg tgcaccacat cgtgaagcag gccctgcagc 1681 gctacagtga ggaccgcatc gggctggcag actacgccct ggagtcagga ggggccagcg 1741 tcatcagcac ccgatgttct gagacctacg agaccaagac ggccctcctc agcctcttcg 1801 gcatccccct gtggtaccac tcccagtcac cccgagtcat cctccagcca gatgtgcacc 1861 caggcaactg ctgggccttc caggggccac aaggcttcgc cgtggTCCGC CTCTCTGCCC 1921 GCATCCGCCC CACAGCCGTT ACCTTAGAGC ATGTGCCCAA GGCCTTGTCA CCCAACAGCA 1981 CTATCTCCAG TGCCCCCAAG GACTTCGCCA TCTTTGGGTT TGACGAAGAC CTGCAGCAGG 2041 AGGGGACACT CCTTGGCAAG TTCACTTACG ATCAGGACGG CGAGCCTATT CAGACGTTTC 2101 ACTTTCAGGC CCCTACGATG GCCACGTACC AGGTGGTGGA GCTGCGGATC CTGACTAACT 2161 GGGGCCACCC CGAGTACACC TGCATCTACC GCTTCAGAGT GCATGGGGAG CCCGCCCACT 2221 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2281 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2341 TTTATATATC TTGTGGAAAG GACGAGAGAA ACCTTTGGCA CTATACGTCA CGCGTTAAGT 2401 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt