Construct: ORF TRCN0000478434
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001581.1_s317c1
- Derived from:
- ccsbBroadEn_01786
- DNA Barcode:
- GACGATGTCTAACTCGCTCGGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XRCC4 (7518)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478434
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7518 | XRCC4 | X-ray repair cross compleme... | NM_001318012.2 | 100% | 100% | |
| 2 | human | 7518 | XRCC4 | X-ray repair cross compleme... | NM_022406.4 | 100% | 100% | |
| 3 | human | 7518 | XRCC4 | X-ray repair cross compleme... | XM_011543626.1 | 100% | 100% | |
| 4 | human | 7518 | XRCC4 | X-ray repair cross compleme... | NM_003401.5 | 99.4% | 99.1% | 893_894insTTCTAG |
| 5 | human | 7518 | XRCC4 | X-ray repair cross compleme... | NM_022550.3 | 99.4% | 99.1% | 893_894insTTCTAG |
| 6 | human | 7518 | XRCC4 | X-ray repair cross compleme... | NM_001318013.2 | 91.4% | 90.1% | (many diffs) |
| 7 | human | 7518 | XRCC4 | X-ray repair cross compleme... | XM_017009828.2 | 91.3% | 89% | (many diffs) |
| 8 | human | 7518 | XRCC4 | X-ray repair cross compleme... | XM_017009827.2 | 90.7% | 89.2% | (many diffs) |
| 9 | human | 7518 | XRCC4 | X-ray repair cross compleme... | XM_017009829.2 | 76.6% | 72.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1074
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagaaaaata agcagaatcc accttgtttc tgaacccagt ataactcatt 121 ttctacaagt atcttgggag aaaacactgg aatctggttt tgttattaca cttactgatg 181 gtcattcagc atggactggg acagtttctg aatcagagat ttcccaagaa gctgatgaca 241 tggcaatgga aaaagggaaa tatgttggtg aactgagaaa agcattgttg tcaggagcag 301 gaccagctga tgtatacacg tttaattttt ctaaagagtc ttgttatttc ttctttgaga 361 aaaacctgaa agatgtctca ttcagacttg gttccttcaa cctagagaaa gttgaaaacc 421 cagctgaagt cattagagaa cttatttgtt attgcttgga caccattgca gaaaatcaag 481 ccaaaaatga gcacctgcag aaagaaaatg aaaggcttct gagagattgg aatgatgttc 541 aaggacgatt tgaaaaatgt gtgagtgcta aggaagcttt ggagactgat ctttataagc 601 ggtttattct ggtgttgaat gagaagaaaa caaaaatcag aagtttgcat aataaattat 661 taaatgcagc tcaagaacga gaaaaggaca tcaaacaaga aggggaaact gcaatctgtt 721 ctgaaatgac tgctgaccga gatccagtct atgatgagag tactgatgag gaaagtgaaa 781 accaaactga TCTCTCTGGG TTGGCTTCAG CTGCTGTAAG TAAAGATGAT TCCATTATTT 841 CAAGTCTTGA TGTCACTGAT ATTGCACCAA GTAGAAAAAG GAGACAGCGA ATGCAAAGAA 901 ATCTTGGGAC AGAACCTAAA ATGGCTCCTC AGGAGAATCA GCTTCAAGAA AAGGAAAATT 961 CTAGGCCTGA TTCTTCACTA CCTGAGACGT CTAAAAAGGA GCACATCTCA GCTGAAAACA 1021 TGTCTTTAGA AACTCTGAGA AACAGCAGCC CAGAAGACCT CTTTGATGAG ATTTACCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAG ACGATGTCTA ACTCGCTCGG ATCACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att