Construct: ORF TRCN0000478438
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008989.2_s317c1
- Derived from:
- ccsbBroadEn_06413
- DNA Barcode:
- TCTCCCACCTAGTGTCGCATCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSP90AA1 (3320)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478438
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3320 | HSP90AA1 | heat shock protein 90 alpha... | NM_005348.4 | 99.9% | 100% | 1080C>T |
| 2 | human | 3320 | HSP90AA1 | heat shock protein 90 alpha... | XM_011536718.2 | 85.7% | 85.8% | 1_363del;1443C>T |
| 3 | human | 3320 | HSP90AA1 | heat shock protein 90 alpha... | NM_001017963.3 | 85.6% | 85.7% | 1_366del;1446C>T |
| 4 | mouse | 15519 | Hsp90aa1 | heat shock protein 90, alph... | NM_010480.5 | 90.1% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2265
- ORF length:
- 2196
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctgaggaa acccagaccc aagaccaacc gatggaggag gaggaggttg 121 agacgttcgc ctttcaggca gaaattgccc agttgatgtc attgatcatc aatactttct 181 actcgaacaa agagatcttt ctgagagagc tcatttcaaa ttcatcagat gcattggaca 241 aaatccggta tgaaagcttg acagatccca gtaaattaga ctctgggaaa gagctgcata 301 ttaaccttat accgaacaaa caagatcgaa ctctcactat tgtggatact ggaattggaa 361 tgaccaaggc tgacttgatc aataaccttg gtactatcgc caagtctggg accaaagcgt 421 tcatggaagc tttgcaggct ggtgcagata tctctatgat tggccagttc ggtgttggtt 481 tttattctgc ttatttggtt gctgagaaag taactgtgat caccaaacat aacgatgatg 541 agcagtacgc ttgggagtcc tcagcagggg gatcattcac agtgaggaca gacacaggtg 601 aacctatggg tcgtggaaca aaagttatcc tacacctgaa agaagaccaa actgagtact 661 tggaggaacg aagaataaag gagattgtga agaaacattc tcagtttatt ggatatccca 721 ttactctttt tgtggagaag gaacgtgata aagaagtaag cgatgatgag gctgaagaaa 781 aggaagacaa agaagaagaa aaagaaaaag aagagaaaga gtcggaagac aaacctgaaa 841 ttgaagatgt tggttctgat gaggaagaag aaaagaagga tggtgacaag aagaagaaga 901 agaagattaa ggaaaagtac atcgatcaag aagagctcaa caaaacaaag cccatctgga 961 ccagaaatcc cgacgatatt actaatgagg agtacggaga attctataag agcttgacca 1021 atgactggga agatcacttg gcagtgaagc atttttcagt tgaaggacag ttggaattca 1081 gagcccttct atttgtccca cgacgtgctc cttttgatct gtttgaaaac agaaagaaaa 1141 agaacaatat caaattgtat gtacgcagag ttttcatcat ggataactgt gaggagctaa 1201 tccctgaata tctgaacttc attagagggg tggtagactc ggaggatctc cctctaaaca 1261 tatcccgtga gatgttgcaa caaagcaaaa ttttgaaagt tatcaggaag aatttggtca 1321 aaaaatgctt agaactcttt actgaactgg cggaagataa agagaactac aagaaattct 1381 atgagcagtt ctctaaaaac ataaagcttg gaatacacga agactctcaa aatcggaaga 1441 agctttcaga gctgttaagg tactacacat ctgcctctgg tgatgagatg gtttctctca 1501 aggactactg caccagaatg aaggagaacc agaaacatat ctattatatc acaggtgaga 1561 ccaaggacca ggtagctaac tcagcctttg tggaacgtct tcggaaacat ggcttagaag 1621 tgatctatat gattgagccc attgatgagt actgtgtcca acagctgaag gaatttgagg 1681 ggaagacttt agtgtcagtc accaaagaag gcctggaact tccagaggat gaagaagaga 1741 aaaagaagca ggaagagaaa aaaacaaagt ttgagaacct ctgcaaaatc atgaaagaca 1801 tattggagaa aaaagttgaa aaggtggttg tgtcaaaccg attggtgaca tctccatgct 1861 gtattgtcac aagcacatat ggctggacag caaacatgga gagaatcatg aaagctcaag 1921 cccTAAGAGA CAACTCAACA ATGGGTTACA TGGCAGCAAA GAAACACCTG GAGATAAACC 1981 CTGACCATTC CATTATTGAG ACCTTAAGGC AAAAGGCAGA GGCTGATAAG AACGACAAGT 2041 CTGTGAAGGA TCTGGTCATC TTGCTTTATG AAACTGCGCT CCTGTCTTCT GGCTTCAGTC 2101 TGGAAGATCC CCAGACACAT GCTAACAGGA TCTACAGGAT GATCAAACTT GGTCTGGGTA 2161 TTGATGAAGA TGACCCTACT GCTGATGATA CCAGTGCTGC TGTAACTGAA GAAATGCCAC 2221 CCCTTGAAGG AGATGACGAC ACATCACGCA TGGAAGAAGT AGACTTGCCA ACTTTCTTGT 2281 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2341 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2401 GAAAGGACGA TCTCCCACCT AGTGTCGCAT CTTAACGCGT TAAGTCgaca atcaacctct 2461 ggattacaaa atttgtgaaa gatt