Construct: ORF TRCN0000478443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011547.1_s317c1
- Derived from:
- ccsbBroadEn_12492
- DNA Barcode:
- TTCTGAGTCTATTTAAATCTCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM15 (64783)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478443
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64783 | RBM15 | RNA binding motif protein 15 | NM_001201545.1 | 99.9% | 99.8% | 679G>A;1542A>T |
| 2 | human | 64783 | RBM15 | RNA binding motif protein 15 | NM_022768.4 | 97.7% | 97.7% | (many diffs) |
| 3 | mouse | 229700 | Rbm15 | RNA binding motif protein 15 | NM_001045807.1 | 89.3% | 93.6% | (many diffs) |
| 4 | mouse | 229700 | Rbm15 | RNA binding motif protein 15 | XR_001783686.1 | 13.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2976
- ORF length:
- 2907
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggactgcg gggcgggacc ctgtgccgcg gcggagtcca agatggcggc 121 gtgcggttcc gctgtgtgaa acgagcgcgg ggcggcgggt tactcagctc cgcggagacg 181 acctccgacg acccgcaaca atgaagggaa aagagcgctc gccagtgaag gccaaacgct 241 cccgtggtgg tgaggactcg acttcccgcg gtgagcggag caagaagtta gggggctctg 301 gtggcagcaa tgggagcagc agcggaaaga ccgatagcgg cggtgggtcg cggcggagtc 361 tccacctgga caagtccagc agtcgaggtg gcagccgcga gtatgatacc ggtgggggca 421 gctccagtag ccgcttgcat agttatagct ccccgagcac caaaaattct tcgggcgggg 481 gcgagtcgcg cagcagctcc cggggtggag gcggggagtc acgttcctct ggggccgcct 541 cctcagctcc cggcggcggg gacggcgcgg aatacaagac tctgaagata agcgagttgg 601 ggtcccagct tagtgacgaa gcggtggagg acggcctgtt tcatgagttc aaacgcttcg 661 gtgatgtaag tgtgaaaatc agtcatctgt cgggttctgg cagcggggat gagcgggtag 721 cctttgtgaa cttccggcgg ccagagaacg cgcgggcggc caagcatgcc agaggccgcc 781 tggtgctcta tgaccggcct ctgaagatag aagctgtgta tgtgagccgg cgccgcagcc 841 gctccccttt agacaaagat acttatcctc catcagccag tgtggtcggg gcctctgtag 901 gtggtcaccg gcacccccct ggaggtggtg gaggccagag atcactttcc cctggtggcg 961 ctgctttggg atacagagac taccggctgc agcagttggc tcttggccgc ctgccccctc 1021 cacctccgcc accattgcct cgagacctgg agagagaaag agactacccg ttctatgaga 1081 gagtgcgccc tgcatacagt cttgagccaa gggtgggagc tggagcaggt gctgctcctt 1141 tcagagaagt ggatgagatt tcacccgagg atgatcagcg agctaaccgg acgctcttct 1201 tgggcaacct agacatcact gtaacggaga gtgatttaag aagggcgttt gatcgctttg 1261 gagtcatcac agaagtagat atcaagaggc cttctcgcgg ccagactagt acttacggct 1321 ttctcaaatt tgagaactta gatatgtctc accgggccaa attagcaatg tctggcaaaa 1381 ttataattcg gaatcctatc aaaattggtt atggtaaagc tacacccacc acccgcctct 1441 gggtgggagg cctgggacct tgggttcctc ttgctgccct ggcacgagaa tttgatcgat 1501 ttggcaccat acgcaccata gactaccgaa aaggtgatag ttgggcatat atccagtatg 1561 aaagcctgga tgcagcgcat gctgcctgga cccatatgcg gggcttccct cttggtggcc 1621 cagatcgacg ccttagagta gactttgccg acaccgaaca tcgttaccag cagcagtatc 1681 tgcagcctct gcccttgact cattatgagc tggtgacaga tgcttttgga catcgggcac 1741 cagacccttt gaggggtgct cgggatagga caccaccctt actatacaga gatcgtgata 1801 gggaccttta tcctgactct gattgggtgc cacccccacc cccagtccga gaacgcagca 1861 ctcggactgc agctacttct gtgcctgctt acgagccact ggatagccta gatcgcaggc 1921 gggatggttg gtccttggac cgggacagag gtgatcgaga tctgcccagc agcagagacc 1981 agcctaggaa gcgaaggctg cctgaggaga gtggaggacg tcatctggat aggtctcctg 2041 agagtgaccg cccacgaaaa cgtcactgcg ctccttctcc tgaccgcagt ccagaattga 2101 gcagtagccg ggatcgttac aacagcgaca atgatcgatc ttcccgtctt ctcttggaaa 2161 ggccctctcc aatcagagac agacgaggta gtttggagaa gagccagggt gacaagcgag 2221 accgtaaaaa ctctgcatca gctgaacgag ataggaagca ccggacaact gctcccactg 2281 agggaaaaag ccctctgaaa aaagaagacc gctctgatgg gagtgcacct agcaccagca 2341 ctgcttcctc caagctgaag tccccgtccc agaaacagga tggggggaca gcccctgtgg 2401 catcagcctc tcccaaactc tgtttggcct ggcagggcat gcttctactg aagaacagca 2461 actttccttc caacatgcat ctgttgcagg gtgacctcca agtggctagt agtcttcttg 2521 tggagggttc aactggaggc aaagtggccc agctcaagat cactcagcgt ctccgtttgg 2581 accagcccaa gttggatgaa gtaactcgac gcatcaaagt agcagggccc aatggttatg 2641 ccattctttt ggctgtgcct ggaagttctg acagccggtc ctcctcttcc TCAGCTGCAT 2701 CAGACACTGC CACTTCTACT CAGAGGCCAC TTAGGAACCT TGTGTCCTAT TTAAAGCAAA 2761 AGCAGGCAGC CGGGGTGATC AGCCTCCCTG TGGGGGGCAA CAAAGACAAG GAAAACACCG 2821 GGGTCCTTCA TGCCTTCCCA CCTTGTGAGT TCTCCCAGCA GTTCCTGGAT TCCCCTGCCA 2881 AGGCACTGGC CAAATCTGAA GAAGATTACC TGGTCATGAT CATTGTCCGT GCAAAACTGG 2941 TTGAACAGCG GATGAAGATA TGGAATTCAA AGCTCTTGCC AACTTTCTTG TACAAAGTGG 3001 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3061 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3121 ATTCTGAGTC TATTTAAATC TCCAAACGCG TTAAGTCgac aatcaacctc tggattacaa 3181 aatttgtgaa agatt