Construct: ORF TRCN0000478455
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000219.1_s317c1
- Derived from:
- ccsbBroadEn_00050
- DNA Barcode:
- GGAAGACGGGCCAGGCCACACTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH3B1 (221)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478455
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_000694.4 | 100% | 100% | |
| 2 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001161473.3 | 100% | 100% | |
| 3 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001030010.3 | 92% | 92% | 161_162ins111 |
| 4 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001290058.2 | 75% | 75% | 476_477ins351 |
| 5 | human | 222 | ALDH3B2 | aldehyde dehydrogenase 3 fa... | NM_001031615.2 | 71.4% | 68.5% | (many diffs) |
| 6 | human | 222 | ALDH3B2 | aldehyde dehydrogenase 3 fa... | NM_001354345.1 | 71.4% | 68.5% | (many diffs) |
| 7 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001290059.2 | 58.1% | 58.1% | 0_1ins588 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1470
- ORF length:
- 1404
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cccccttggg gacacgctgc ggcgactgcg ggaggccttc cacgcggggc 121 gcacgcggcc agctgagttc cgggctgcgc agctccaagg cctgggccgc ttcctgcaag 181 aaaacaagca gcttctgcac gacgcactgg cccaggacct gcacaagtca gccttcgagt 241 cggaggtgtc tgaggttgcc atcagccagg gcgaggtcac cctggccctc aggaacctcc 301 gggcctggat gaaggacgag cgtgtgccca agaacctggc cacgcagctg gactccgcct 361 tcatccggaa ggagcccttt ggcctggtcc tcatcattgc gccctggaac tatccgctga 421 acctgacgct ggtgcccctc gtgggagccc tcgctgcagg gaactgtgtg gtgctgaagc 481 catcggagat tagcaagaac gtcgagaaga tcctggccga ggtgctgccc caatacgtgg 541 accagagctg ctttgctgtg gtgctgggcg ggccccagga gacggggcag ctgctagagc 601 acaggttcga ctacatcttc ttcacaggga gccctcgtgt gggcaagatt gttatgactg 661 ctgccgccaa gcacctgaca cctgtcaccc tggagctggg gggcaagaac ccttgctacg 721 tggacgacaa ctgcgacccc cagaccgtgg ccaaccgcgt ggcctggttc cgctacttca 781 acgccggcca gacctgcgtg gcccccgact acgtcctatg cagccctgag atgcaggaga 841 ggctgctgcc tgccctgcag agcaccatca cccgtttcta tggcgacgac ccccagagct 901 ccccaaacct gggccgcatc atcaaccaga aacagttcca gcggctgcgg gcattgctgg 961 gctgcggccg tgtggccatt gggggccaga gcgatgagag cgatcgctac atcgccccca 1021 cggtgctggt ggatgtgcag gagatggagc ctgtgatgca ggaggagatc ttcgggccca 1081 tcctgcccat cgtgaacgtg cagagcttgg acgaggccat cgagttcatc aaccggcggg 1141 agaagcccct ggccctgtac gccttcTCCA ACAGCAGCCA GGTGGTCAAG CGGGTGCTGA 1201 CCCAGACCAG CAGCGGGGGC TTCTGTGGGA ACGACGGCTT CATGCACATG ACCCTGGCCA 1261 GCCTGCCTTT TGGAGGAGTG GGTGCCAGTG GGATGGGCCG GTACCATGGC AAGTTCTCCT 1321 TCGACACCTT CTCCCACCAT CGCGCCTGCC TCCTGCGCAG CCCGGGGATG GAGAAGCTCA 1381 ACGCCCTCCG CTACCCGCCG CAATCGCCGC GCCGCCTGAG GATGCTGCTG GTGGCCATGG 1441 AGGCCCAAGG CTGCAGCTGC ACACTGCTCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGAAG 1621 ACGGGCCAGG CCACACTTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt