Construct: ORF TRCN0000478455

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000219.1_s317c1
Derived from:
ccsbBroadEn_00050
DNA Barcode:
GGAAGACGGGCCAGGCCACACTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALDH3B1 (221)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478455
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 221 ALDH3B1 aldehyde dehydrogenase 3 fa... NM_000694.4 100% 100%
2 human 221 ALDH3B1 aldehyde dehydrogenase 3 fa... NM_001161473.3 100% 100%
3 human 221 ALDH3B1 aldehyde dehydrogenase 3 fa... NM_001030010.3 92% 92% 161_162ins111
4 human 221 ALDH3B1 aldehyde dehydrogenase 3 fa... NM_001290058.2 75% 75% 476_477ins351
5 human 222 ALDH3B2 aldehyde dehydrogenase 3 fa... NM_001031615.2 71.4% 68.5% (many diffs)
6 human 222 ALDH3B2 aldehyde dehydrogenase 3 fa... NM_001354345.1 71.4% 68.5% (many diffs)
7 human 221 ALDH3B1 aldehyde dehydrogenase 3 fa... NM_001290059.2 58.1% 58.1% 0_1ins588
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1470
ORF length:
1404
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cccccttggg gacacgctgc ggcgactgcg ggaggccttc cacgcggggc
121gcacgcggcc agctgagttc cgggctgcgc agctccaagg cctgggccgc ttcctgcaag
181aaaacaagca gcttctgcac gacgcactgg cccaggacct gcacaagtca gccttcgagt
241cggaggtgtc tgaggttgcc atcagccagg gcgaggtcac cctggccctc aggaacctcc
301gggcctggat gaaggacgag cgtgtgccca agaacctggc cacgcagctg gactccgcct
361tcatccggaa ggagcccttt ggcctggtcc tcatcattgc gccctggaac tatccgctga
421acctgacgct ggtgcccctc gtgggagccc tcgctgcagg gaactgtgtg gtgctgaagc
481catcggagat tagcaagaac gtcgagaaga tcctggccga ggtgctgccc caatacgtgg
541accagagctg ctttgctgtg gtgctgggcg ggccccagga gacggggcag ctgctagagc
601acaggttcga ctacatcttc ttcacaggga gccctcgtgt gggcaagatt gttatgactg
661ctgccgccaa gcacctgaca cctgtcaccc tggagctggg gggcaagaac ccttgctacg
721tggacgacaa ctgcgacccc cagaccgtgg ccaaccgcgt ggcctggttc cgctacttca
781acgccggcca gacctgcgtg gcccccgact acgtcctatg cagccctgag atgcaggaga
841ggctgctgcc tgccctgcag agcaccatca cccgtttcta tggcgacgac ccccagagct
901ccccaaacct gggccgcatc atcaaccaga aacagttcca gcggctgcgg gcattgctgg
961gctgcggccg tgtggccatt gggggccaga gcgatgagag cgatcgctac atcgccccca
1021cggtgctggt ggatgtgcag gagatggagc ctgtgatgca ggaggagatc ttcgggccca
1081tcctgcccat cgtgaacgtg cagagcttgg acgaggccat cgagttcatc aaccggcggg
1141agaagcccct ggccctgtac gccttcTCCA ACAGCAGCCA GGTGGTCAAG CGGGTGCTGA
1201CCCAGACCAG CAGCGGGGGC TTCTGTGGGA ACGACGGCTT CATGCACATG ACCCTGGCCA
1261GCCTGCCTTT TGGAGGAGTG GGTGCCAGTG GGATGGGCCG GTACCATGGC AAGTTCTCCT
1321TCGACACCTT CTCCCACCAT CGCGCCTGCC TCCTGCGCAG CCCGGGGATG GAGAAGCTCA
1381ACGCCCTCCG CTACCCGCCG CAATCGCCGC GCCGCCTGAG GATGCTGCTG GTGGCCATGG
1441AGGCCCAAGG CTGCAGCTGC ACACTGCTCT GCCCAACTTT CTTGTACAAA GTGGTTGATA
1501TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1561CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGAAG
1621ACGGGCCAGG CCACACTTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1681tgaaagatt

Download FASTA (ORF) (Full)