Construct: ORF TRCN0000478486
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004778.1_s317c1
- Derived from:
- ccsbBroadEn_06312
- DNA Barcode:
- TCATTGCTGGCTATCCGTTGTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR20 (2843)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478486
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2843 | GPR20 | G protein-coupled receptor 20 | NM_005293.3 | 99.7% | 99.7% | 465C>T;624C>T;689A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1143
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccctctgtg tctccagcgg ggccctcggc cggggcagtc cccaatgcca 121 ccgcagtgac aacagtgcgg accaatgcca gcgggctgga ggtgcccctg ttccacctgt 181 ttgcccggct ggacgaggag ctgcatggca ccttcccagg cctgtggctg gcgctgatgg 241 cggtgcacgg agccatcttc ctggcagggc tggtgctcaa cgggctggcg ctgtacgtct 301 tctgctgccg cacccgggcc aagacaccct cagtcatcta caccatcaac ctggtggtga 361 ccgatctact ggtagggctg tccctgccca cgcgcttcgc tgtgtactac ggcgccaggg 421 gctgcctgcg ctgtgccttc ccgcacgtcc tcggttactt cctcaacatg cactgctcca 481 tcctcttcct cacctgcatc tgcgtggacc gctacctggc catcgtgcgg cctgaaggct 541 cccgccgctg ccgccagcct gcctgtgcca gggccgtgtg cgccttcgtg tggctggccg 601 ccggtgccgt caccctgtcg gtgctgggcg tgacaggcag ccggccctgc tgccgtgtct 661 ttgcgctgac tgtcctggag ttcctgctgc ctctgctggt catcagcgtg tttaccggcc 721 gcatcatgtg tgcactgtcg cggccgggtc tgctccgcca gggtcgccag cgccgcgtgc 781 gggccatgca gcTCCTGCTC ACGGTGCTCA TCATCTTTCT CGTCTGCTTC ACGCCCTTCC 841 ACGCCCGCCA AGTGGCCGTG GCGCTGTGGC CCGACATGCC ACACCACACG AGCCTCGTGG 901 TCTACCACGT GGCCGTGACC CTCAGCAGCC TCAACAGCTG CATGGACCCC ATCGTCTACT 961 GCTTCGTCAC CAGTGGCTTC CAGGCCACCG TCCGAGGCCT CTTCGGCCAG CACGGAGAGC 1021 GTGAGCCCAG CAGCGGTGAC GTGGTCAGCA TGCACAGGAG CTCCAAGGGC TCAGGCCGTC 1081 ATCACATCCT CAGTGCCGGC CCTCACGCCC TCACCCAGGC CCTGGCTAAT GGGCCCGAGG 1141 CTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGATC ATTGCTGGCT ATCCGTTGTC GCACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt