Construct: ORF TRCN0000478500
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015461.1_s317c1
- Derived from:
- ccsbBroadEn_05719
- DNA Barcode:
- CTCCGAGTAGCACGTCAACTTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCNI2 (645121)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478500
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 645121 | CCNI2 | cyclin I family member 2 | NM_001039780.4 | 100% | 100% | |
| 2 | human | 645121 | CCNI2 | cyclin I family member 2 | NM_001287253.2 | 99.7% | 99.7% | 427_429delCAG |
| 3 | human | 645121 | CCNI2 | cyclin I family member 2 | NM_001287252.1 | 95.8% | 95.8% | 775_822del |
| 4 | human | 645121 | CCNI2 | cyclin I family member 2 | XM_017009725.2 | 78.9% | 78.9% | 427_429delCAG;777_778ins231 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1176
- ORF length:
- 1107
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctcgggc gctcagctcc cgccgcagcc gtcgagctca gaggtcagcg 121 ccgtccagag cccaggcggg cgtcccggcg ccggtctgga ggaaacagcc ctgggcgttc 181 ctctcccgcc gtctccgggg gaggcccctc tgccccgaag caaccggagc aggtgccctg 241 ggacccgcca gcccggagcg gcctccctcc acgcggcgtc cgcagcagtc cccgtgcggc 301 cccggcgcgg tacggcgcca gccgggaaaa ccgcagacgc ggtccccgcc gccgccccag 361 agcaagctcc gcggccggct ccacagtccc gcaagccgcg caacctggaa ggcgacctgg 421 acgagcgccg gctgctctgc cacttgcagc tggcccagga ccgcgaggcg cgcctgtggc 481 ggggcggcaa accccaggat gaaatctgcg acgccttcga ggaagtcgtg ctgtggctcc 541 tgcggcttca gaacaccttt tacttctccc agtccacttt taacctggcc ctcaccatct 601 ttggccgcct cctgatttca gtgaaggtaa aagagaaata cctgcattgc gccacaatta 661 cttccttgag gctcgctgca aaagttaatg aagaagagga gtttattcca caagtaaaag 721 acttcacaaa gcactatggc tctgactatt ccccgaatga gctgctgagg atggagctgg 781 ctattctgga cagactgcac tgggacctct atattgggac gccgctggac ttcttgacta 841 tattccatgc cctggtggtc ctgagctggc cccatgtgtt GGAGCTGCTG CCTCAGAGGA 901 ATCCTTCCCT CCACGTCGCA TCCCTGACCA GGCAGCTGCA GCACTGTATG GCGGGCCACC 961 AGCTGCTGCA GTTCAAGGGC TCCACACTGG CCTTGGTCAT CATCACCTTA GAGCTGGAGA 1021 GGCTCATGCC CGGCTGGTGT GCTCCTATAT CTGATCTGCT AAAGAAAGCA CAGGTTGGTG 1081 ATATGCAGTA CAGCTGCTGC AAGGAACTTG TAATGCAGCA ACTGAGAAGT CTTCAGTCAT 1141 CCTCCTGCAC AGACAACTTT GTGTCACCTG CCAACTTGCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 ACTCCGAGTA GCACGTCAAC TTAGGACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt