Construct: ORF TRCN0000478512
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001159.1_s317c1
- Derived from:
- ccsbBroadEn_08391
- DNA Barcode:
- ACTTAGCCGCCCATGAGCCCGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL50 (54534)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478512
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54534 | MRPL50 | mitochondrial ribosomal pro... | NM_019051.3 | 99.5% | 99.3% | 379C>T;435C>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgcgatct gtgtcgggca ttaccagaag agtcttcatg tggacagtct 121 cagggacacc atgtagagaa ttttggtctc gatTCAGAAA AGAGAAAGAG CCAGTGGTTG 181 TTGAGACAGT AGAAGAGAAA AAGGAACCTA TCCTAGTGTG TCCACCTTTA CGAAGCCGAG 241 CATACACACC ACCTGAAGAT CTCCAGAGTC GTTTGGAATC TTACGTTAAA GAAGTTTTTG 301 GTTCATCTCT TCCTAGTAAT TGGCAAGACA TCTCCCTGGA AGATAGTCGT CTAAAGTTCA 361 ATCTTCTGGC TCATTTAGCT GATGACTTGG GTCATGTAGT CCCTAACTCC AGACTCCACC 421 AGATGTGCAG GGTTAGAGAT GTTTTTGATT TCTATAATGT CCCTATTCAA GATAGATCTA 481 AATTTGATGA ACTCAGTGCG AGTAATCTGC CCCCCAATTT GAAAATCACT TGGAGTTACT 541 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TTGTGGAAAG GACGAACTTA GCCGCCCATG AGCCCGTTCA CGCGTTAAGT 721 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt