Construct: ORF TRCN0000478514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015805.1_s317c1
- Derived from:
- ccsbBroadEn_01655
- DNA Barcode:
- TACGGTATAATCTTCTACTAGTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNLT3 (6990)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478514
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6990 | DYNLT3 | dynein light chain Tctex-ty... | NM_006520.3 | 100% | 100% | |
| 2 | mouse | 67117 | Dynlt3 | dynein light chain Tctex-ty... | NM_025975.5 | 86.4% | 90.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 414
- ORF length:
- 348
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatGGA GGAGTACCAT CGCCACTGCG ACGAGGTTGG CTTCAATGCT GAGGAAGCCC 121 ACAATATTGT CAAAGAGTGT GTAGATGGGG TTTTAGGTGG TGAAGATTAT AATCACAACA 181 ACATCAACCA GTGGACTGCA AGCATAGTGG AACAATCCTT AACACACCTG GTTAAGTTGG 241 GAAAAGCCTA TAAATATATT GTGACCTGTG CAGTGGTCCA GAAGAGCGCA TATGGCTTTC 301 ACACAGCCAG CTCCTGTTTT TGGGATACCA CATCTGATGG AACCTGTACC GTAAGATGGG 361 AGAACCGGAC CATGAACTGT ATTGTCAACG TTTTTGCCAT TGCTATTGTT CTTTACCCAA 421 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 481 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 541 TATCTTGTGG AAAGGACGAT ACGGTATAAT CTTCTACTAG TGTACGCGTT AAGTCgacaa 601 tcaacctctg gattacaaaa tttgtgaaag att