Construct: ORF TRCN0000478525
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010185.1_s317c1
- Derived from:
- ccsbBroadEn_03123
- DNA Barcode:
- TTTACGTAGTGTCCCCCGATCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC39A3 (29985)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478525
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29985 | SLC39A3 | solute carrier family 39 me... | NM_213568.2 | 100% | 100% | |
| 2 | human | 29985 | SLC39A3 | solute carrier family 39 me... | NM_144564.5 | 29.8% | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 381
- ORF length:
- 315
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGT GAAATTGCTA GTGGCCAAAA TCCTGTGCAT GGTGGGCGTG TTCTTCTTCA 121 TGCTGCTCGG CTCCCTGCTC CCCGTGAAGA TCATCGAGAC AGATTTTGAG AAGGCCCATC 181 GCTCGAAAAA GATCCTCTCT CTCTGCAACA CCTTTGGAGG AGGGGTGTTT CTGGCCACGT 241 GCTTCAACGC TCTGCTGCCC GCTGTGAGGG AAAAGGTAAG GGCTCCCTGG GCACTAGCAG 301 CAGCCCTTGG CACCTTATGG CCAAGGGACT CTGATGCATT TTCAACACTG ATGCCAAGTT 361 CAGTGAAGGC CTTGATGCTG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTTA CGTAGTGTCC 541 CCCGATCCGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt