Construct: ORF TRCN0000478539
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000649.1_s317c1
- Derived from:
- ccsbBroadEn_09032
- DNA Barcode:
- CCTGGCATCTCTAAGTCCTTGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BPIFB2 (80341)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478539
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80341 | BPIFB2 | BPI fold containing family ... | NM_025227.3 | 99.8% | 99.5% | 770T>G;1361T>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1440
- ORF length:
- 1374
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttgggcaagt aggctgggcc tgctgctggc actgctgctg cccgtggtcg 121 gtgcctccac gccaggcacc gtggtccgac tcaacaaggc agcattgagc tacgtgtctg 181 aaattgggaa agcccctctc cagcgggccc tgcaggtcac tgtccctcat ttcctggact 241 ggagtggaga ggcgcttcag cccaccagga tccggattct gaatgtccat gtgccccgcc 301 tccacctgaa attcattgct ggtttcggag tgcgcctgct ggcagcagct aattttactt 361 tcaaggtctt tcgcgcccca gagcccctgg agctgacgct gcctgtggaa ctgctggctg 421 acacccgcgt gacccagagc tccatcagga cccctgtggt cagcatctct gcctgctctt 481 tattctcggg ccacgccaac gagtttgatg gcagtaacag cacctcccac gcgctgctgg 541 tcctggtgca gaagcacatt aaagctgtct tgagtaacaa gctgtgcctg agcatctcca 601 acctggtgca gggtgtcaat gtccacctgg gcaccttaat tggcctcaac cccgtgggtc 661 ctgagtccca gatccgctat tccatggtca gtgtgcccac tgtcaccagt gactacattt 721 ccctggaagt caatgctgtt ctcttcctgc tgggcaagcc catcatcctg cccacggatg 781 ccaccccttt tgtgttgcca aggcatgtgg gtaccgaggg ctccatggcc accgggggcc 841 tctcccagca gctgtttgac tctgcgctcc tgctgctgca gaaggccggt gccctcaacc 901 tggacatcac agggcagctg aggtcggatg acaacctgct gaacacctct gctctgggcc 961 ggctcatccc ggaggtggcc cgccagtttc ccgagcccat gcctgtggtg ctcaaggtgc 1021 ggctgggtgc cacacctgtg gccatgctcc acacaaacaa cgccaccctg cggctgcagc 1081 ccttcgtgga ggtcctggcc acagcctcca actcggcttt ccagtccctc ttctccctGG 1141 ATGTGGTAGT GAACTTGAGA CTCCAGCTCT CTGTGTCCAA GGTGAAGCTT CAGGGGACCA 1201 CGTCTGTGCT GGGGGATGTC CAGCTCACGG TGGCCTCCTC CAACGTGGGC TTCATTGATA 1261 CAGATCAGGT GCGCACACTG ATGGGCACCG TTTTTGAGAA GCCCCTGCTG GACCATCTCA 1321 ATGCTCTCTT GGCCATGGGA ATTGCCCTCC CTGGTGTGGT CAACCTCCAC TATGTCGCCC 1381 CTGAGATCTT TGTCTATGAG GGCTACGTGG TGATATCCAG TGGACACTTC TACCAGAGCT 1441 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1501 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1561 TTTATATATC TTGTGGAAAG GACGACCTGG CATCTCTAAG TCCTTGACTA CGCGTTAAGT 1621 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt