Construct: ORF TRCN0000478549
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002017.1_s317c1
- Derived from:
- ccsbBroadEn_01802
- DNA Barcode:
- GTTCGAAACTAATAGTCGCCCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MZF1 (7593)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478549
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7593 | MZF1 | myeloid zinc finger 1 | NM_003422.2 | 100% | 100% | |
| 2 | human | 7593 | MZF1 | myeloid zinc finger 1 | NM_198055.2 | 100% | 100% | |
| 3 | human | 7593 | MZF1 | myeloid zinc finger 1 | XM_011527264.3 | 96% | 96% | 1_90del |
| 4 | human | 7593 | MZF1 | myeloid zinc finger 1 | XM_005259204.3 | 94.7% | 94.7% | 1_123del |
| 5 | human | 7593 | MZF1 | myeloid zinc finger 1 | XM_024451689.1 | 94.7% | 94.7% | 1_123del |
| 6 | human | 7593 | MZF1 | myeloid zinc finger 1 | XM_017027206.1 | 61.3% | 61.3% | 0_1ins852 |
| 7 | human | 7593 | MZF1 | myeloid zinc finger 1 | NM_001267033.2 | 39.5% | 36.5% | 766_767ins244;870_871ins1088 |
| 8 | mouse | 109889 | Mzf1 | myeloid zinc finger 1 | NM_001290452.1 | 62.1% | 63.3% | (many diffs) |
| 9 | mouse | 109889 | Mzf1 | myeloid zinc finger 1 | NM_001290453.1 | 54.9% | 57.3% | (many diffs) |
| 10 | mouse | 109889 | Mzf1 | myeloid zinc finger 1 | XM_011250413.2 | 45.6% | 49.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2268
- ORF length:
- 2202
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcctgcggtg ctgggctccc cagaccgagc acccccagaa gatgaggggc 121 ctgtcatggt gaagctagag gactctgagg aggagggtga ggctgcctta tgggacccag 181 gccctgaagc tgcacgcctg cgtttccggt gcttccgcta tgaggaggcc acagggcccc 241 aagaggccct ggcccagctc cgagagctgt gtcgccagtg gctgcgtcca gaggtacgct 301 ccaaggagca gatgctggag ctgttggtgc tggagcagtt cctgggcgca ctgccccctg 361 agatccaggc ccgtgtgcag gggcagcggc caggcagccc cgaggaggct gctgccctag 421 tagatgggct gcgccgggag ccgggcggac cccggagatg ggtcacagtc caggtgcagg 481 gccaggaggt cctatcagag aagatggagc cctccagttt ccagccccta cctgaaactg 541 agcctccaac tccagagcct gggcccaaga cacctcctag gactatgcag gaatcaccac 601 tgggcctgca ggtgaaagag gagtcagagg ttacagagga ctcagatttc ctggagtctg 661 ggcctctagc tgccacccag gagtctgtac ccaccctcct gcctgaggag gcccagagat 721 gtgggaccgt gctggaccag atctttcccc acagcaagac tgggcctgag ggtccctcat 781 ggagggagca ccccagggcc ctgtggcatg aggaagctgg gggcatcttc tccccagggt 841 tcgcgctgca gctaggcagc atctccgcag gtccaggtag tgtaagccct cacctccacg 901 tcccctggga cctcggcatg gctggccttt ctggccagat ccaatcaccc tcccgcgaag 961 gtggctttgc gcatgcgctt ctgctcccca gcgatctgag gagtgaacag gaccccacgg 1021 acgaggatcc ctgccggggt gtgggccctg ctctgatcac cacccgctgg cgctccccca 1081 ggggccggag ccggggccgc cccagcactg ggggcggggt ggttaggggc ggccgttgcg 1141 atgtatgtgg caaggtgttc agccaacgca gcaacctgct gaggcaccag aagatccaca 1201 cgggtgagcg accattcgtg tgcagcgagt gcggccgcag cttcagccgc agctcgcacc 1261 tgctgcgcca ccagcttacg cacaccgagg agcggccgtt cgtgtgcggc gactgtggcc 1321 agggcttcgt gcgcagcgcg cgcctggaag agcatcggag agtgcacacg ggcgaacagc 1381 ctttccgttg cgctgagtgc ggccagagct tccggcagcg ctccaatctg ctgcagcacc 1441 agcgcatcca cggcgatccc ccgggccctg gcgctaagcc cccggcccct cctggtgcgc 1501 ccgagcctcc cggccccttt ccgtgcagcg agtgccgcga gagcttcgcg cggcgcgccg 1561 tgctgctgga gcaccaggcg gtacacacgg gcgacaagtc ctttggctgc gtcgagtgcg 1621 gcgagcgctt cggccgccgc tcagtgctgc tgcagcaccg gcgcgtgcac agtggcgagc 1681 ggcccttcgc ctgtgccgag tgcggccaga gcttccggca gcgctccaac ctgacgcagc 1741 accggcgcat ccacaccggg gagcggccct tcgcctgcgc cgagtgtggc aaggccttcc 1801 gccagcggcc tacgctcacg cagcatctcc gcgtacacac gggcgagaaa ccctttgcct 1861 gccccgagtg tggccagcgc ttcagccagc gcctcaagct cacgcgtcaT CAGAGGACAC 1921 ACACCGGCGA AAAGCCCTAC CACTGCGGTG AGTGCGGCCT GGGCTTCACG CAGGTCTCGC 1981 GGCTCACCGA GCACCAGCGC ATCCACACGG GCGAACGGCC CTTCGCCTGC CCCGAGTGCG 2041 GCCAGAGCTT TCGGCAGCAC GCCAACCTCA CCCAGCACCG GCGCATCCAC ACGGGTGAAC 2101 GGCCCTACGC ATGCCCTGAG TGTGGCAAGG CCTTCCGCCA GCGGCCCACG CTCACGCAGC 2161 ATCTGCGCAC CCACCGACGA GAGAAGCCCT TCGCCTGCCA GGACTGTGGC CGCCGCTTCC 2221 ACCAGAGCAC CAAGCTCATT CAGCACCAGC GCGTCCACAG CGCCGAGTAC CCAACTTTCT 2281 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2341 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2401 GTGGAAAGGA CGAGTTCGAA ACTAATAGTC GCCCTTTACG CGTTAAGTCg acaatcaacc 2461 tctggattac aaaatttgtg aaagatt