Construct: ORF TRCN0000478566
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006806.2_s317c1
- Derived from:
- ccsbBroadEn_06609
- DNA Barcode:
- GTCACGTAATGTCGCCCCGTTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYL6 (4637)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478566
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4637 | MYL6 | myosin light chain 6 | NM_021019.5 | 99.7% | 100% | 453G>C |
| 2 | human | 4637 | MYL6 | myosin light chain 6 | NM_079423.4 | 97.3% | 96.6% | (many diffs) |
| 3 | mouse | 17904 | Myl6 | myosin, light polypeptide 6... | NM_001317218.1 | 95.3% | 100% | (many diffs) |
| 4 | mouse | 17904 | Myl6 | myosin, light polypeptide 6... | NM_010860.4 | 93.3% | 96.6% | (many diffs) |
| 5 | mouse | 17904 | Myl6 | myosin, light polypeptide 6... | NM_001317217.1 | 89.5% | 91.7% | (many diffs) |
| 6 | mouse | 17904 | Myl6 | myosin, light polypeptide 6... | NM_001317219.1 | 72.8% | 76.8% | (many diffs) |
| 7 | mouse | 17904 | Myl6 | myosin, light polypeptide 6... | NM_001317220.1 | 70.9% | 73.5% | (many diffs) |
| 8 | mouse | 102635661 | LOC102635661 | myosin light polypeptide 6-... | XM_006531565.3 | 70.6% | 72.8% | (many diffs) |
| 9 | mouse | 100043172 | Gm10080 | predicted gene 10080 | XR_140938.4 | 68.9% | (many diffs) | |
| 10 | mouse | 17904 | Myl6 | myosin, light polypeptide 6... | XM_006513323.2 | 68.1% | 69.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgacttcacc gaagaccaga ccgcagagtt caaggaggcc TTCCAGCTGT 121 TTGACCGAAC AGGTGATGGC AAGATCCTGT ACAGCCAGTG TGGGGATGTG ATGAGGGCCC 181 TGGGCCAGAA CCCTACCAAC GCCGAGGTGC TCAAGGTCCT GGGGAACCCC AAGAGTGATG 241 AGATGAATGT GAAGGTGCTG GACTTTGAGC ACTTTCTGCC CATGCTGCAG ACAGTGGCCA 301 AGAACAAGGA CCAGGGCACC TATGAGGATT ATGTCGAAGG ACTTCGGGTG TTTGACAAGG 361 AAGGAAATGG CACCGTCATG GGTGCTGAAA TCCGGCATGT TCTTGTCACA CTGGGTGAGA 421 AGATGACAGA GGAAGAAGTA GAGATGCTGG TGGCAGGGCA TGAGGACAGC AATGGTTGTA 481 TCAACTATGA AGCGTTTGTG AGGCATATCC TGTCGGGCTG CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGAGTCACG TAATGTCGCC CCGTTCGTAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt