Construct: ORF TRCN0000478572
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011729.1_s317c1
- Derived from:
- ccsbBroadEn_07210
- DNA Barcode:
- CCGGAAACAGCCCAGACCCCGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNPLA4 (8228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478572
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8228 | PNPLA4 | patatin like phospholipase ... | NM_001142389.1 | 99.7% | 99.2% | 143T>G;401A>G |
| 2 | human | 8228 | PNPLA4 | patatin like phospholipase ... | NM_004650.3 | 99.7% | 99.2% | 143T>G;401A>G |
| 3 | human | 8228 | PNPLA4 | patatin like phospholipase ... | XM_011545588.1 | 99.7% | 99.2% | 143T>G;401A>G |
| 4 | human | 8228 | PNPLA4 | patatin like phospholipase ... | NM_001172672.1 | 65.4% | 65.2% | 0_1ins261;140A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcacatcaac ctatcatttg cagcgtgtgg atttctgggc atttaccact 121 tgggggcagc atctgcactt tgcagacatg gcaaaaaact tgtgaaggat gtcaaagcct 181 tcgctggggc gtctgcggga tcgttgggtg cttctgttct gctaacagca ccagaaaaaa 241 tagaggaatg taaccaattt acctacaagt ttgccgaaga aatcagaagg cagtctttcg 301 gggcagtaac gcccggttat gacttcatgg cccgactaag aagtgggatg gagtcgattc 361 ttcctcccag cgctcacgag ctggcccaga accgactgca cgtatccatc accaacgcca 421 aaaccagaga aaatcactta gtctccactt tttcctccag ggagggcctc attaaggtcc 481 TCCTAGCCAG CAGTTTTGTG CCCATTTATG CAGGACTGAA GCTAGTGGAA TACAAAGGGC 541 AGAAGTGGGT GGACGGAGGC CTCACCAACG CTCTTCCCAT CCTGCCCGTC GGCCGGACAG 601 TAACCATCTC CCCCTTCAGT GGACGACTGG ACATCTCCCC GCAGGACAAA GGGCAGCTAG 661 ATCTGTATGT TAATATCGCC AAGCAGGATA TCATGTTGTC CCTGGCAAAC CTGGTGAGAC 721 TCAACCAAGC CCTTTTTCCC CCAAGCAAGA GGAAAATGGA ATCTTTGTAT CAGTGTGGTT 781 TTGATGACAC TGTTAAGTTT TTACTTAAAG AAAATTGGTT TGAATACCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA CCGGAAACAG CCCAGACCCC GTGGACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt