Construct: ORF TRCN0000478580
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005028.1_s317c1
- Derived from:
- ccsbBroadEn_10130
- DNA Barcode:
- CAAGCGTCTCATAAAATGGGCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLJ44955 (401278)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478580
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 104472716 | WARS2-IT1 | WARS2 intronic transcript 1 | NR_126447.1 | 22.3% | (many diffs) | |
| 2 | human | 440594 | LINC01761 | long intergenic non-protein... | NR_033966.1 | 18.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 672
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctttgacc aaaatgctga taatgatatg gacaatgaaa tccaggctga 121 gctggtctca gatggagatg aggaacttgt tgggaattgg agctaaagtg actcgttatg 181 ttttagcaaa gagactggtg gcattttgcc cctgccctaa agatttgtgg aactttgaac 241 ttgagagaga tgatttaggg tatctggtgg aagaagtttc TAAGCAGCAA AGCATTCAAG 301 AGGTGACTTG GGTGCTGTTA AAGGCATTCA GTTTTATAAG GGAAGCAGAG CATAAAAGGT 361 TGGAAAATTT GCAGCCTGAT AATGTGATAG AAAAGAAAAT CCTATTTTCT GAGGAGAAAT 421 TTAAGCTGGC TGCAAAAATT TGGATAAGTA ATGAGGAGCT GAATATTAAT CCCCAAGACA 481 ATGGGGAAAA TGTCTGCAGG GCATGTCAGA GGTCTTCACA GCAGCTCCTC CTGTCACAGG 541 CCTGGAGGCC TAGAAGGAAA AAATGGTTTC ATGGTCTGGG CCCAAGGCCC CCTTGCTCTG 601 TGCAGCCTAA GGACTTGGTG CCTTGCGTCC CAGCCACTCC AGCCATGGCT AAAAGGGGCC 661 AAGGTACAGT TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 721 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 781 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAA GCGTCTCATA AAATGGGCAT 841 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t