Construct: ORF TRCN0000478582
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003497.1_s317c1
- Derived from:
- ccsbBroadEn_10986
- DNA Barcode:
- TGTATTTATACTCCTTCTGAGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYT1 (4661)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478582
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4661 | MYT1 | myelin transcription factor 1 | NM_004535.3 | 51.6% | 50.5% | (many diffs) |
| 2 | mouse | 17932 | Myt1 | myelin transcription factor 1 | XM_006500581.3 | 56% | 59.2% | (many diffs) |
| 3 | mouse | 17932 | Myt1 | myelin transcription factor 1 | XM_011239932.2 | 53.4% | 56.2% | (many diffs) |
| 4 | mouse | 17932 | Myt1 | myelin transcription factor 1 | XR_375392.3 | 51.9% | (many diffs) | |
| 5 | mouse | 17932 | Myt1 | myelin transcription factor 1 | NM_008665.4 | 46.2% | 47.7% | (many diffs) |
| 6 | mouse | 17932 | Myt1 | myelin transcription factor 1 | XM_006500577.3 | 46.2% | 47.7% | (many diffs) |
| 7 | mouse | 17932 | Myt1 | myelin transcription factor 1 | XM_006500580.3 | 45% | 45.4% | (many diffs) |
| 8 | mouse | 17932 | Myt1 | myelin transcription factor 1 | NM_001171615.1 | 44.5% | 46% | (many diffs) |
| 9 | mouse | 17932 | Myt1 | myelin transcription factor 1 | XM_006500578.3 | 43.9% | 45.4% | (many diffs) |
| 10 | mouse | 17932 | Myt1 | myelin transcription factor 1 | NM_001171616.1 | 43.2% | 44.7% | (many diffs) |
| 11 | mouse | 17932 | Myt1 | myelin transcription factor 1 | XM_006500579.3 | 43.2% | 44.7% | (many diffs) |
| 12 | mouse | 17932 | Myt1 | myelin transcription factor 1 | XR_001781155.1 | 25.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1839
- ORF length:
- 1773
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cttagaaaat gaagacaagc gagctcgcac ccgatccaag gccctgcgag 121 gacccccaga gaccacagct gcagacctca gctgccccac cccaggatgc acaggctcag 181 ggcacgtccg gggcaagtac tccaggcacc gaagtttaca gagctgcccc ctggccaaga 241 agaggaagct ggagggcgct gaggctgagc acctggtgtc caagaggaag tcacaccccc 301 tgaagctggc tctggacgag ggctatggtg tggacagcga cggcagtgag gacactgagg 361 tgaaggacgc ctctgtttcg gatgaatcgg aaggaactct ggagggggcc gaggctgaga 421 cgtcaggaca ggacgagatt catcgccccg agacagctga agatccttca agagctgaga 481 agcgtgagat caagtgtcca acaccaggct gtgatggcac tggccacgtt accgggttgt 541 accctcacca ccgcagcctt tctggctgtc cccacaagga taggatcccc ccagagatct 601 tagccatgca tgagaacgtg ctgaagtgcc ccactcctgg ctgcacaggc cagggtcacg 661 tgaacagcaa ccgcaacacg cacagaagtt tgtctgggtg tcccattgct gccgccgaaa 721 aattagccaa atcccatgag aagcagcagc cgcagacagg agatccttcc aagagtagct 781 ccaattccga tcggatcctc aggcccatgt gcttcgtgaa gcagctcgag gtccctccat 841 atgggagcta ccggcccaac gtggcccccg ccacacccag ggccaacttg gccaaggagc 901 tggagaagtt ctccaaggtc acctttgact acgcaagttt cgatgctcag gtttttggca 961 aacgcatgct tgccccaaag attcagacca gcgaaacctc acctaaagcc tttcaatgct 1021 ttgactactc gcaggacgcc gaggctgcac acatggctgc cactgccatc ctgaacctct 1081 ccacgcgctg ctgggagatg cctgagaacc tcagcacgaa gccacaggac ctccccagca 1141 agtctgtgga tatcgaggta gacgaaaatg gaaccctgga cttgagcatg cacaaacacc 1201 gcaaacgaga aaatgctttc cccagcagca gcagctgcag cagcagcccc ggtgtgaagt 1261 ctcccgacgc ctcccagcgc cacagcagca ccagcgcccc cagcagctcc atgacctctc 1321 cccagtccag ccaggcctcc cgccaggacg agtgggaccg gcccctggac tacaccaagc 1381 ctagccgcct gagagaggag gaacctgagg agtcagagcc agcagcccat tcttttgctt 1441 cttcTGAAGC AGATGACCAG GAAGTGTCGG AAGAGAATTT TGAGGAGCGG AAGTATCCGG 1501 GGGAAGTCAC CCTGACCAAC TTTAAGCTGA AGTTTCTCTC CAAGGACATA AAGAAGGAGC 1561 TGCTCACCTG TCCCACCCCT GGCTGTGACG GCAGCGGCCA CATCACCGGG AACTACGCCT 1621 CCCACCGCAG GTGCCCCACG CCCGGCTGTG ACGGCTCTGG CCACATCACA GGGAACTACG 1681 CTTCACACCG GAGCTTGTCC GGCTGCCCTC GTGCAAAGAA AAGTGGAGTC AAGGTGGCAC 1741 CCACCAAGGA CGACAAGGAG GACCCCGAGC TGATGAAGTA CGTTGGGCCA TGCTGGCTCT 1801 TTCATTGCAT TGCGGAATTG AGATTTTCGT GTGTTTTATA CCCAACTTTC TTGTACAAAG 1861 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1921 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1981 ACGATGTATT TATACTCCTT CTGAGAATAC GCGTTAAGTC gacaatcaac ctctggatta 2041 caaaatttgt gaaagatt