Construct: ORF TRCN0000478592
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003656.1_s317c1
- Derived from:
- ccsbBroadEn_11089
- DNA Barcode:
- TAAGTCCTAACTACTGTTCTTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASGRF1 (5923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478592
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5923 | RASGRF1 | Ras protein specific guanin... | XM_017022459.2 | 95.4% | 95.2% | (many diffs) |
| 2 | human | 5923 | RASGRF1 | Ras protein specific guanin... | XM_011521867.3 | 94.4% | 94.2% | (many diffs) |
| 3 | human | 5923 | RASGRF1 | Ras protein specific guanin... | XM_017022458.2 | 92.5% | 90.7% | (many diffs) |
| 4 | human | 5923 | RASGRF1 | Ras protein specific guanin... | XM_017022457.2 | 92.3% | 90.5% | (many diffs) |
| 5 | human | 5923 | RASGRF1 | Ras protein specific guanin... | NM_001145648.2 | 92.1% | 90.1% | (many diffs) |
| 6 | human | 5923 | RASGRF1 | Ras protein specific guanin... | XM_017022456.2 | 91.9% | 89.8% | (many diffs) |
| 7 | human | 5923 | RASGRF1 | Ras protein specific guanin... | XM_011521866.3 | 91.4% | 89.5% | (many diffs) |
| 8 | human | 5923 | RASGRF1 | Ras protein specific guanin... | XM_017022455.2 | 91.1% | 89.1% | (many diffs) |
| 9 | human | 5923 | RASGRF1 | Ras protein specific guanin... | NM_002891.5 | 90.9% | 88.9% | (many diffs) |
| 10 | human | 5923 | RASGRF1 | Ras protein specific guanin... | NM_153815.2 | 31.4% | 29.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3609
- ORF length:
- 3543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gaaggggatc cggctgaatg atggccacgt cgcgtccctg ggactgctgg 121 cgcgcaagga cggcacgcgc aaaggctacc tgagcaagcg gagttcggac aacacaaaat 181 ggcaaaccaa gtggttcgcg ctgctgcaga acctgctctt ctacttcgag agcgactcga 241 gctcgcggcc ctcggggctt tacctgctgg agggctgcgt ctgcgaccgc gcgccctccc 301 ccaagccggc gctgtcggcc aaggagccgc tggagaaaca gcattacttc acggtgaact 361 tcagccatga gaaccagaaa gccttggagc tgaggacaga ggacgcaaaa gattgtgacg 421 aatgggtggc agccattgca catgccagct acaggaccct cgccacagag catgaggcat 481 taatgcagaa atacctgcac ctgctgcaga tcgtggagac agagaagacc gtggccaagc 541 agcttcggca gcagatcgag gatggggaga tcgagatcga gcggctgaag gcagagatca 601 catccctgct caaggacaat gagcgcatcc agtccaccca gactgtcgcc cccaacgatg 661 aagacagcga catcaagaaa attaagaagg tgcagagctt cctgcggggc tggctgtgcc 721 ggcggaagtg gaagaccatc atccaggact acatccggtc accccatgct gacagcatgc 781 gcaagaggaa ccaggtggtg ttcagcatgc tggaggctga ggctgagtac gtgcagcagc 841 tgcacatcct tgtcaacaat ttcctgcgcc cgctgcggat ggccgccagc tccaagaagc 901 ctcccatcac acacgacgac gtcagcagca tcttcctgaa cagcgaaacc atcatgtttt 961 tacatcagat cttttaccaa ggcctgaagg cccgcatctc cagctggccc acgctggtcc 1021 tggctgacct atttgacatc ctgctgccca tgctcaacat ctaccaagag ttcgtccgca 1081 accaccagta cagcctgcag atcctggccc actgcaagca gaaccgtgac ttcgacaagc 1141 tgctgaagca ctacgaggcc aagcctgact gcgaggagag gacgctggag accttcctca 1201 cctaccccat gttccagatc cccaggtaca tcctgaccct ccatgagctc ctggcccaca 1261 cgcctcatga gcacgttgag cgcaacagcc tggactacgc caagtccaaa ctggaggagc 1321 tgtccagaat aatgcacgat gaagtaagtg agacggagaa catccggaaa aacctggcca 1381 tcgagcgcat gatcatcgaa ggctgtgaga tcctcctgga caccagccag acctttgtga 1441 gacaaggttc cctcattcag gtgcccatgt ctgaaaaggg caagatcacc agggggcgcc 1501 tggggtctct ctccctaaag aaagagggcg agcgacagtg cttcctgttt tctaagcatc 1561 tgattatctg taccagaggc tctggaggga agcttcactt gaccaagaat ggagtcatat 1621 ccctcattga ctgcacttta ttggaggagc cagaaagcac ggaggaggaa gccaaaggat 1681 ccggccaaga catagatcac ttggatttta aaatcggggt ggagccaaag gattccccgc 1741 cctttacagt catcctagtg gcctcgtcca gacaggagaa ggcagcgtgg accagtgaca 1801 tcagccagtg tgtggataac atccgatgca atgggctcat gatgaacgca tttgaagaaa 1861 attccaaggt cactgtgccg cagatgatca agtccgacgc ctccttatat tgtgatgatg 1921 ttgacattcg cttcagcaaa accatgaact cctgcaaagt gctgcagatc cgctacgcca 1981 gtgtggagcg gctgctggag aggctgacgg acctgcgctt cctgagcatc gacttcctca 2041 acaccttcct gcactcctac cgcgtcttca ccaccgccat cgtggtcctg gacaagctca 2101 ttaccatcta caagaagcct atcagtgcca ttcctgccag gtcgctggag ctcctgtttg 2161 ccagtggcca gaacaataag ctcctgtacg gtgaaccccc caagtccccg cgcgccaccc 2221 gcaagttctc ctcgccgcca cctctgtcca tcaccaagac atcgtcaccg agccgccggc 2281 ggaagctctc cctgaacatc cccatcatca ctggcggcaa ggccctggac ctggccgccc 2341 tcagctgcaa ctccaatggc tacaccagca tgtactcggc catgtcaccc ttcagcaagg 2401 ccacgctgga caccagcaag ctctatgtgt ccagcagctt caccaacaag attccagatg 2461 agggcgatac gacccctgag aagcccgaag acccttcagc gctcagcaag cagagctcag 2521 aagtctccat gagagaggag tcagatattg atcaaaacca gagtgatgat ggtgatactg 2581 aaacatcacc aactaaatct ccaacaacac ccaaatcagt caaaaacaaa aattcttcag 2641 agttcccact cttttcctat aacaatggag tcgtcatgac ctcctgtcgt gaactggaca 2701 ataaccgcag tgccttgtcg gccgcctctg cctttgccat agcaaccgcc ggggccaacg 2761 agggcacccc aaacaaggag aagtaccgga ggatgtcctt agccagtgca gggtttcccc 2821 cagaccagag gaatggagac aaggagtttg tgatccgcag agcagccacc aatcgtgtct 2881 tgaacgtgct ccgccactgg gtgtccaagc actctcagga ctttgagacc aacgatgagc 2941 tcaaatgcaa ggtgatcggc ttcctggaag aagtcatgca cgacccggag ctcctgaccc 3001 aggagcggaa ggctgcagcc aacatcatca ggactctgac ccaggaggac ccaggtgaca 3061 accagatcac gctggaggag atcacgcaga tggctgaagg cgtgaaggct gagccctttg 3121 aaaaccactc agccctggag atcgcggagc agctgaccct gctagatcac ctcgtcttca 3181 agaagattcc ttatgaggag ttcttcggac aaggatggat gaaactggaa aagaatgaaa 3241 ggacccctta tatcatgaaa accacTAAGC ACTTCAATGA CATCAGTAAC TTGATTGCTT 3301 CAGAAATCAT CCGCAACGAG GACATCAACG CCAGGGTGAG CGCCATCGAG AAGTGGGTGG 3361 CCGTAGCTGA CATATGCCGC TGCCTCCACA ACTACAATGC CGTACTGGAG ATCACCTCGT 3421 CCATGAACCG CAGTGCAATC TTCCGGCTCA AAAAGACGTG GCTCAAAGTC TCTAAGCAGG 3481 TGCGGGCAGG AGGCTGGCAC CACTGCCTCC CTGGGGCTGG ATTGGGGCTG GGGCAGGAAG 3541 ACCCGTGGGC TGGCCACTGG GCATCCACAA GTGGGCAAAG GACTGGGCTG AGGTCTAACA 3601 CTCCCAGGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAAGTC CTAACTACTG TTCTTCATAC 3781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt