Construct: ORF TRCN0000478601
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000512.1_s317c1
- Derived from:
- ccsbBroadEn_02638
- DNA Barcode:
- TCATGTTACCATGCAAAAGTGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT3 (11165)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478601
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11165 | NUDT3 | nudix hydrolase 3 | NM_006703.4 | 100% | 100% | |
| 2 | human | 100529239 | RPS10-NUDT3 | RPS10-NUDT3 readthrough | NM_001202470.2 | 54.5% | 49.3% | (many diffs) |
| 3 | mouse | 56409 | Nudt3 | nudix (nucleotide diphospha... | NM_019837.2 | 88.9% | 95.3% | (many diffs) |
| 4 | mouse | 56409 | Nudt3 | nudix (nucleotide diphospha... | NM_001291046.1 | 71.8% | 76.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gaagctcaag tcgaaccaga cccgcaccta cgacggcgac ggctacaaga 121 agcgGGCCGC ATGCCTGTGT TTCCGCAGCG AGAGCGAGGA GGAGGTGCTA CTCGTGAGCA 181 GTAGTCGCCA TCCAGACAGA TGGATTGTCC CTGGAGGAGG CATGGAGCCC GAGGAGGAGC 241 CAAGTGTGGC AGCAGTTCGT GAAGTCTGTG AGGAGGCTGG AGTAAAAGGG ACATTGGGAA 301 GATTAGTTGG AATTTTTGAG AACCAGGAGA GGAAGCACAG GACGTATGTC TATGTGCTCA 361 TTGTCACTGA AGTGCTGGAA GACTGGGAAG ATTCAGTTAA CATTGGAAGG AAGAGGGAAT 421 GGTTTAAAAT AGAAGACGCC ATAAAAGTGC TGCAGTATCA CAAACCCGTG CAGGCATCAT 481 ATTTTGAAAC ATTGAGGCAA GGCTACTCAG CCAACAATGG CACCCCAGTC GTGGCCACCA 541 CATACTCGGT TTCTGCTCAG AGCTCGATGT CAGGCATCAG ATGCCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGATCA TGTTACCATG CAAAAGTGAT GACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t