Construct: ORF TRCN0000478604

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016227.3_s317c1
Derived from:
ccsbBroadEn_12008
DNA Barcode:
TAGTTCATAAAGTCCTGTACGTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NLK (51701)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478604
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51701 NLK nemo like kinase NM_016231.5 97.6% 97.5% 1_36del;73G>A
2 human 51701 NLK nemo like kinase XM_005257988.2 92.1% 92% 1_36del;73G>A;1149_1150ins87
3 human 51701 NLK nemo like kinase XR_934482.1 72% 1_263del;300G>A;1809_2144del
4 human 51701 NLK nemo like kinase XR_001752526.2 62.6% (many diffs)
5 mouse 18099 Nlk nemo like kinase NM_008702.3 92.1% 97.5% (many diffs)
6 mouse 18099 Nlk nemo like kinase XR_001779914.1 31% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1611
ORF length:
1545
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggcttacaat ggcggtacat ctgcagcagc aacaggtcac caccaccacc
121atcaccacca ccttccacac ctccctcctc ctcacctgca ccaccaccac caccctcaac
181accatcttca tccggggtcg gctgccgctg tacaccctgt acagcagcac acctcttcgg
241cagctgcggc agccgcagca gcggctgcag ctgcagccat gttaaaccct gggcaacaac
301agccatattt cccatcaccg gcaccggggc aggctcctgg accagctgca gcagccccag
361ctcaggtaca ggctgccgca gctgctacag ttaaggcgca ccatcatcag cactcgcatc
421atccacagca gcagctggat attgagccgg atagacctat tggatatgga gcctttggtg
481ttgtctggtc agtaacagat ccaagagatg gaaagagagt agcgctcaaa aagatgccca
541acgtcttcca gaatctggtc tcttgcaaaa gggtcttccg ggaattgaag atgttgtgtt
601tttttaagca tgataatgta ctctctgccc ttgacatact ccaacctcca cacattgact
661attttgaaga aatatatgtt gtcacagaat tgatgcagag tgacctacat aaaattatcg
721tctctcctca accactcagc tcagatcatg tcaaagtttt tctttatcag attttgcgag
781gtttgaaata tctccattca gctggcattt tacatcgaga cattaagcca gggaatctcc
841ttgtgaacag caactgtgtt ctaaagattt gtgattttgg attggccaga gtggaagaat
901tagatgaatc ccgtcatatg actcaggaag ttgttactca gtattatcgg gctccagaaa
961tcctgatggg cagccgtcat tacagcaatg ctattgacat ctggtctgtg ggatgtatct
1021ttgcagaact actaggacga agaatattgt ttcaggcaca gagtcccatt cagcagttgg
1081atttgatcac ggatctgttg ggcacaccat cactggaagc aatgaggaca gcttgtgaag
1141gcgctaaggc acatatactc aggggtcctc ataaacagcc atctcttcct gtactctata
1201ccctgtctag ccaggctaca catgaagctg ttcaTCTCCT TTGCAGGATG TTGGTCTTTG
1261ATCCATCCAA AAGAATATCC GCTAAGGATG CCTTAGCCCA CCCCTACCTA GATGAAGGGC
1321GACTACGATA TCACACATGT ATGTGTAAAT GTTGCTTTTC CACCTCCACT GGAAGAGTTT
1381ATACCAGTGA CTTTGAGCCT GTCACCAATC CCAAATTTGA TGACACTTTC GAGAAGAACC
1441TCAGTTCTGT CCGACAGGTT AAAGAAATTA TTCATCAGTT CATTTTGGAA CAGCAGAAAG
1501GAAACAGAGT GCCTCTCTGC ATCAACCCTC AGTCTGCTGC TTTTAAGAGC TTTATTAGTT
1561CCACTGTTGC TCAGCCATCT GAGATGCCCC CATCTCCTCT GGTGTGGGAG TGCCCAACTT
1621TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1681CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1741CTTGTGGAAA GGACGATAGT TCATAAAGTC CTGTACGTTG ACGCGTTAAG TCgacaatca
1801acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)