Construct: ORF TRCN0000478623
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008131.1_s317c1
- Derived from:
- ccsbBroadEn_02829
- DNA Barcode:
- ATACTGCGGGGCTCACACCGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMPX (23676)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478623
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23676 | SMPX | small muscle protein X-linked | NM_014332.3 | 100% | 100% | |
| 2 | human | 23676 | SMPX | small muscle protein X-linked | NR_045617.2 | 26.7% | 1_187del;452_987del | |
| 3 | mouse | 66106 | Smpx | small muscle protein, X-linked | NM_001252591.2 | 82.1% | 84% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 330
- ORF length:
- 264
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGAA TATGTCGAAA CAGCCAGTTT CCAATGTTAG AGCCATCCAG GCAAATATCA 121 ATATTCCAAT GGGAGCCTTT CGGCCAGGAG CAGGTCAACC CCCCAGAAGA AAAGAATGTA 181 CTCCTGAAGT GGAGGAGGGT GTTCCTCCCA CCTCGGATGA GGAGAAGAAG CCAATTCCAG 241 GAGCGAAGAA ACTTCCAGGA CCTGCAGTCA ATCTATCGGA AATCCAGAAT ATTAAAAGTG 301 AACTAAAATA TGTCCCCAAA GCTGAACAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 361 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 421 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATACT 481 GCGGGGCTCA CACCGGCGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 541 tgaaagatt