Construct: ORF TRCN0000478630
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003976.1_s317c1
- Derived from:
- ccsbBroadEn_07443
- DNA Barcode:
- GTTCACCTTGATCCTGGTGCCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V1G1 (9550)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478630
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9550 | ATP6V1G1 | ATPase H+ transporting V1 s... | NM_004888.4 | 99.7% | 100% | 345C>T |
| 2 | mouse | 66290 | Atp6v1g1 | ATPase, H+ transporting, ly... | NM_024173.2 | 91.2% | 94.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 444
- ORF length:
- 354
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCTAGTCA GTCTCAGGGG ATTCAGCAGC 121 TGCTGCAGGC CGAGAAGCGG GCAGCCGAGA AGGTGTCCGA GGCCCGCAAA AGAAAGAACC 181 GGAGGCTGAA GCAGGCCAAA GAAGAAGCTC AGGCTGAAAT TGAACAGTAC CGCCTGCAGA 241 GGGAGAAAGA ATTCAAGGCC AAGGAAGCTG CGGCATTGGG ATCCCGTGGC AGTTGCAGCA 301 CTGAAGTGGA GAAGGAGACC CAGGAGAAGA TGACCATCCT CCAGACATAC TTCCGGCAGA 361 ACAGGGATGA AGTCTTGGAC AACCTCTTGG CTTTTGTCTG TGACATTCGG CCAGAAATCC 421 ATGAAAACTA CCGTATAAAT GGATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TTCACCTTGA 601 TCCTGGTGCC AGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att