Construct: ORF TRCN0000478632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000735.1_s317c1
- Derived from:
- ccsbBroadEn_16141
- DNA Barcode:
- CGGTACCAACAGGCCTAATCACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN5P2 (260294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168348.2 | 28.5% | 27.8% | (many diffs) |
| 2 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_018044.4 | 26% | 25.4% | (many diffs) |
| 3 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_148956.4 | 23.9% | 23.3% | (many diffs) |
| 4 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168347.3 | 23.7% | 23.1% | (many diffs) |
| 5 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XR_242255.5 | 20.5% | (many diffs) | |
| 6 | human | 260294 | NSUN5P2 | NSUN5 pseudogene 2 | NR_033323.3 | 19.6% | 1_1112del;1463_1784delinsG | |
| 7 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_033322.3 | 19.4% | (many diffs) | |
| 8 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_104013.1 | 18.4% | (many diffs) | |
| 9 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XM_005277604.5 | 15.9% | 6.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 86
- ORF end:
- 437
- ORF length:
- 351
- Sequence:
-
1 tcttcGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT TTTTTATAAT 61 GCCAACTTTG TACAAAAAAG TTGGCATGCC GAGCAGACAG CTGGAGGAGC CCGGGGCAGG 121 GACACCTAGC CCGGTGCGTC TGCATGCCCT GGCAGGGTTC CAGCAGCGAG CCCTGTGCCA 181 CGCGCTCACT TTCCCTTCCC TGCAGCGGCT CGTCTACTCC ATGTGCTCCC TCTGCCAGGA 241 GGAGAATGAA GACATGGTAC AAGATGCGCT GCAGCAGAAC CCGGGCGCCT TCAGGCTAGC 301 TCCCGCCCTG CCTGCCCGGC CCCACCGAGG CCTGAGCACG TTCCCGGGTG CCGAGCACTG 361 CCTCCGGGCT TCCCCCAAGA CCACGCTTAG CGGTGGCTTC TTCGTTGCTG TAATTGAACG 421 GGTCGAGATG CCGACGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 481 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 541 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGGTACCA ACAGGCCTAA 601 TCACGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt