Construct: ORF TRCN0000478633
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017019.1_s317c1
- Derived from:
- ccsbBroadEn_02367
- DNA Barcode:
- CAAGCCAACACCCTGTAAATATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALCOCO2 (10241)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478633
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10241 | CALCOCO2 | calcium binding and coiled-... | NM_005831.5 | 100% | 100% | |
| 2 | human | 10241 | CALCOCO2 | calcium binding and coiled-... | NM_001261391.2 | 95.5% | 95.5% | 181_243del |
| 3 | human | 10241 | CALCOCO2 | calcium binding and coiled-... | NM_001261390.2 | 94.8% | 94.8% | 181_252del |
| 4 | human | 10241 | CALCOCO2 | calcium binding and coiled-... | NM_001261393.2 | 90.5% | 90.5% | 414_415ins126 |
| 5 | human | 10241 | CALCOCO2 | calcium binding and coiled-... | NM_001261395.2 | 83.8% | 83.8% | 0_1ins216 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1404
- ORF length:
- 1338
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagaccatc aaagatcccc ccacatcagc tgtcttgctg gatcactgtc 121 atttctctca ggtcatcttt aacagtgtgg agaagttcta catccctgga ggggacgtca 181 catgtcatta taccttcacc cagcatttca tccctcgtcg aaaggattgg attggcatct 241 ttagagtggg gtggaagaca acccgtgagt attacacctt catgtgggtt actttgccca 301 ttgacctaaa caacaaatca gctaaacagc aggaagtcca attcaaagct tactacctgc 361 ccaaggatga tgagtattac cagttctgct atgtggatga ggatggtgtg gtccggggag 421 caagtattcc tttccaattc cgtccagaaa atgaggaaga catcctggtt gttaccactc 481 agggagaggt ggaagagatt gagcagcaca acaaggagct ttgcaaagaa aaccaggagc 541 tgaaggacag ctgtatcagc ctccagaagc agaactcaga catgcaggct gagctccaaa 601 agaagcagga ggagctagaa accctacaga gcatcaataa gaagttggaa ctgaaagtga 661 aagaacagaa ggactattgg gagacagagc tgcttcaact gaaagaacaa aaccagaaga 721 tgtcctcaga aaatgagaag atgggaatca gagtggatca gcttcaggcc cagctgtcaa 781 ctcaagagaa agaaatggag aagcttgttc agggagatca agataagaca gagcagttag 841 agcagctgaa aaaggaaaat gaccacctct ttctcagttt aactgaacag aggaaggacc 901 agaagaagct cgagcagaca gtggagcaaa tgaagcagaa tgaaactact gcaatgaaga 961 aacaacagga attaatggat gaaaactttg acctgtcaaa aagactgagt gagaacgaaa 1021 ttatatgtaa tgctctgcag agacagaaag agagattgga aggagaaaat gaTCTTTTGA 1081 AGAGGGAGAA CAGCAGATTG CTCAGTTACA TGGGTCTGGA TTTTAATTCT TTGCCGTATC 1141 AAGTACCTAC TTCAGATGAA GGAGGCGCAA GACAAAATCC AGGACTTGCC TATGGAAACC 1201 CATATTCTGG TATCCAAGAA AGTTCTTCCC CCAGCCCGCT CTCCATCAAG AAATGCCCTA 1261 TCTGCAAAGC AGATGATATT TGTGATCACA CCTTGGAGCA ACAGCAGATG CAGCCCCTTT 1321 GTTTCAATTG TCCAATTTGT GACAAGATCT TCCCAGCTAC AGAGAAGCAG ATCTTTGAAG 1381 ACCACGTGTT CTGCCACTCT CTCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1441 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1501 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC AAGCCAACAC 1561 CCTGTAAATA TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1621 att