Construct: ORF TRCN0000478643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016749.1_s317c1
- Derived from:
- ccsbBroadEn_02775
- DNA Barcode:
- CCGACTAAGTTCATAGCATATAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC11A (23478)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478643
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23478 | SEC11A | SEC11 homolog A, signal pep... | NM_014300.4 | 100% | 100% | |
| 2 | human | 23478 | SEC11A | SEC11 homolog A, signal pep... | NM_001271918.1 | 85.4% | 85.4% | 0_1ins78 |
| 3 | human | 23478 | SEC11A | SEC11 homolog A, signal pep... | NM_001271920.1 | 78.9% | 78.1% | 432_469del;492_493ins83 |
| 4 | human | 23478 | SEC11A | SEC11 homolog A, signal pep... | NM_001271922.1 | 78.1% | 71.7% | 430_431ins58;480_555del |
| 5 | human | 23478 | SEC11A | SEC11 homolog A, signal pep... | NM_001271919.1 | 77.6% | 77.6% | 311_312ins120 |
| 6 | human | 23478 | SEC11A | SEC11 homolog A, signal pep... | NM_001271921.1 | 72% | 71.5% | 161_162ins150 |
| 7 | human | 23478 | SEC11A | SEC11 homolog A, signal pep... | NR_073518.1 | 36.1% | 1_385del;817_878del;985_1486del | |
| 8 | mouse | 56529 | Sec11a | SEC11 homolog A, signal pep... | NM_019951.1 | 90.8% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 603
- ORF length:
- 537
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gtctctagac tttttggacg atgtgcggcg gatgaacaag cggcagctct 121 attatcaagt cctaaatttt ggaatgattg tctcatcggc actaatgatc tggaaggggt 181 taatggtaat aactggaagt gaaagtccga ttgtagtggt gctcagtggc agcatggaac 241 ctgcatttca tagaggagaT CTTCTCTTTC TAACAAATCG AGTTGAAGAT CCCATACGAG 301 TGGGAGAAAT TGTTGTTTTT AGGATAGAAG GAAGAGAGAT TCCTATAGTT CACCGAGTCT 361 TGAAGATTCA TGAAAAGCAA AATGGGCATA TCAAGTTTTT GACCAAAGGA GATAATAATG 421 CGGTTGATGA CCGAGGCCTC TATAAACAAG GACAACATTG GCTAGAGAAA AAAGATGTTG 481 TGGGGAGAGC CAGGGGATTT GTTCCTTATA TTGGAATTGT GACGATCCTC ATGAATGACT 541 ATCCTAAATT TAAGTATGCA GTTCTCTTTT TGCTGGGTTT ATTCGTGCTG GTTCATCGTG 601 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 661 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 721 GGCTTTATAT ATCTTGTGGA AAGGACGACC GACTAAGTTC ATAGCATATA GCACGCGTTA 781 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt