Construct: ORF TRCN0000478658
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002873.2_s317c1
- Derived from:
- ccsbBroadEn_06618
- DNA Barcode:
- CACTATGAGGAGATTCAAACCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NBN (4683)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478658
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4683 | NBN | nibrin | NM_002485.4 | 99.7% | 99.7% | (many diffs) |
| 2 | human | 4683 | NBN | nibrin | NM_001024688.2 | 88.9% | 88.8% | (many diffs) |
| 3 | human | 4683 | NBN | nibrin | XM_011517045.1 | 88.9% | 88.8% | (many diffs) |
| 4 | human | 4683 | NBN | nibrin | XM_024447163.1 | 88.9% | 88.8% | (many diffs) |
| 5 | human | 4683 | NBN | nibrin | XM_024447164.1 | 88.9% | 88.8% | (many diffs) |
| 6 | human | 4683 | NBN | nibrin | XM_011517046.1 | 61.9% | 61.4% | (many diffs) |
| 7 | human | 4683 | NBN | nibrin | XM_017013460.1 | 61% | 61% | (many diffs) |
| 8 | human | 4683 | NBN | nibrin | XM_017013462.2 | 61% | 61% | (many diffs) |
| 9 | human | 4683 | NBN | nibrin | XM_024447165.1 | 61% | 61% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2331
- ORF length:
- 2262
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggaaactg ctgcccgccg cgggcccggc aggaggagaa ccatacagac 121 ttttgactgg cgttgagtac gttgttggaa ggaaaaactg tgccattcta attgaaaatg 181 atcagtcgat cagccgaaat catgctgtgt taactgctaa cttttctgta accaacctga 241 gtcaaacaga tgaaatccct gtattgacat taaaagataa ttctaagtat ggtacctttg 301 ttaatgagga aaaaatgcag aatggctttt cccgaacttt gaagtcgggg gatggtatta 361 cttttggagt gtttggaagt aaattcagaa tagagtatga gcctttggtt gcatgctctt 421 cttgtttaga tgtctctggg aaaactgctt taaatcaagc tatattgcaa cttggaggat 481 ttactgtaaa caattggaca gaagaatgca ctcaccttgt catggtatca gtgaaagtta 541 ccattaaaac aatatgtgca ctcatttgtg gacgtccaat tgtaaagcca gaatatttta 601 ctgaattcct gaaagcagtt cagtccaaga agcagcctcc acaaattgaa agtttttacc 661 cacctcttga tgaaccatct attggaagta aaaatgttga tctgtcagga cggcaggaaa 721 gaaaacaaat cttcaaaggg aaaacattta tatttttgaa tgccaaacag cataagaaat 781 tgagttccgc agttgtcttt ggaggtgggg aagctaggtt gataacagaa gagaatgaag 841 aagaacataa tttctttttg gctccgggaa cgtgtgttgt tgatacagga ataacaaact 901 cacagacctt aattcctgac tgtcagaaga aatggattca gtcaataatg gatatgctcc 961 aaaggcaagg tcttagacct attcctgaag cagaaattgg attggcggtg attttcatga 1021 ctacaaagaa ttactgtgat cttcagggcc atcccagtac aggattaaag acaacaactc 1081 caggaccaag cctttcacaa ggcgtgtcag ttgatgaaaa actaatgcca agcgccccag 1141 tgaacactac aacatacgta gctgacacag aatcagagca agcagataca tgggatttga 1201 gtgaaaggcc aaaagaaatc aaagtctcca aaatggaaca aaaattcaga atgctttcac 1261 aagacgcacc cactgtaaag gagtcctgca aaacaagctc taataataat agtatggtat 1321 caaatacttt ggctaagatg agaatcccaa actatcagct ttcaccaact aaattgccaa 1381 gtataaataa aagtaaagat agggcttctc agcagcagca gaccaactcc atcagaaact 1441 actttcagcc gtctaccaaa aaaagggaaa gggatgaaga aaatcaagaa atgtcttcat 1501 gcaaatcagc aagaatagaa acgtcttgtt ctcttttaga acaaacacaa cctgctacac 1561 cctcattgtg gaaaaataag gagcagcatc tatctgagaa tgagcctgtg gacacaaact 1621 cagacaataa cttatttaca gatacagatt taaaatctat tgtgaaaaat tctgccagta 1681 aatctcatgc tgcagaaaag ctaagatcaa ataaaaaaag ggaaatggat gatgtggcca 1741 tagaagatga agtattggaa cagttattca aggacacaaa accagagtta gaaattgatg 1801 tgaaagttca aaaacaggag gaagatgtca atgttagaaa aaggccaagg atggatatag 1861 aaacaaatga cactttcagt gatgaagcag taccagaaag tagcaaaata tctcaagaaa 1921 atgaaattGG GAAGAAACGT GAACTCAAGG AAGACTCACT ATGGTCAGCT AAAGAAATAT 1981 CTAACAATGA CAAACTTCAG GATGATAGTG AGATGCTTCC AAAAAAGCTG TTATTGACTG 2041 AATTTAGATC ACTGGTGATT AAAAACTCTA CTTCCAGAAA TCCGTCTGGC ATAAATGATG 2101 ATTATGGTCA ACTAAAAAAT TTCAAGAAAT TCAAAAAGGT CACATATCCT GGAGCAGGAA 2161 AACTTCCACA CATCATTGGA GGATCAGATC TAATAGCTCA TCATGCTCGA AAGAATACAG 2221 AACTAGAAGA GTGGCTAAGG CAGGAAATGG AGGTACAAAA TCAACATGCA AAAGAAGAGT 2281 CTCTTGCTGA TGATCTTTTT AGATACAATC CTTATTTAAA AAGGAGAAGA TTGCCAACTT 2341 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2401 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2461 CTTGTGGAAA GGACGACACT ATGAGGAGAT TCAAACCTTA ACGCGTTAAG TCgacaatca 2521 acctctggat tacaaaattt gtgaaagatt