Construct: ORF TRCN0000478667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005365.1_s317c1
- Derived from:
- ccsbBroadEn_09980
- DNA Barcode:
- GGAATCTGCACGCAGGCCGTTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP4V2 (285440)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285440 | CYP4V2 | cytochrome P450 family 4 su... | NM_207352.4 | 99.8% | 99.8% | 775C>A;810T>G |
| 2 | human | 285440 | CYP4V2 | cytochrome P450 family 4 su... | XM_005262935.4 | 99.6% | 99.6% | 775C>A;810T>G;1224_1225insGCA |
| 3 | human | 285440 | CYP4V2 | cytochrome P450 family 4 su... | XM_017008037.1 | 74.7% | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1641
- ORF length:
- 1575
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggggctctgg ctggggctcg tgtggcagaa gctgctgctg tggggcgcgg 121 cgagtgccct ttccctggcc ggcgccagtc tggtcctgag cctgctgcag agggtggcga 181 gctacgcgcg gaaatggcag cagatgcggc ccatccccac ggtggcccgc gcctacccac 241 tggtgggcca cgcgctgctg atgaagccgg acgggcgaga attttttcag cagatcattg 301 agtacacaga ggaataccgc cacatgccgc tgctgaagct ctgggtcggg ccagtgccca 361 tggtggccct ttataatgca gaaaatgtgg aggtaatttt aactagttca aagcaaattg 421 acaaatcctc tatgtacaag tttttagaac catggcttgg cctaggactt cttacaagta 481 ctggaaacaa atggcgctcc aggagaaaga tgttaacacc cactttccat tttaccattc 541 tggaagattt cttagatatc atgaatgaac aagcaaatat attggttaag aaacttgaaa 601 aacacattaa ccaagaagca tttaactgct ttttttacat cactctttgt gccttagata 661 tcatctgtga aacagctatg gggaagaata ttggtgctca aagtaatgat gattccgagt 721 atgtccgtgc agtttataga atgagtgaga tgatatttcg aagaataaag atgccctggc 781 tttggcttga tctctggtac cttatgttta aagaaggatg ggaacacaaa aagagcctta 841 agatcctaca tacttttacc aacagtgtca tcgcggaacg ggccaatgaa atgaacgcca 901 atgaagactg tagaggtgat ggcaggggct ctgccccctc caaaaataaa cgcagggcct 961 ttcttgactt gcttttaagt gtgactgatg acgaagggaa caggctaagt catgaagata 1021 ttcgagaaga agttgacacc ttcatgtttg aggggcacga tacaactgca gctgcaataa 1081 actggtcctt atacctgttg ggttctaacc cagaagtcca gaaaaaagtg gatcatgaat 1141 tggatgacgt gtttgggaag tctgaccgtc ccgctacagt agaagacctg aagaaacttc 1201 ggtatctgga atgtgttatt aaggagaccc ttcgcctttt tccTTCTGTT CCTTTATTTG 1261 CCCGTAGTGT TAGTGAAGAT TGTGAAGTGG CAGGTTACAG AGTTCTAAAA GGCACTGAAG 1321 CCGTCATCAT TCCCTATGCA TTGCACAGAG ATCCGAGATA CTTCCCCAAC CCCGAGGAGT 1381 TCCAGCCTGA GCGGTTCTTC CCCGAGAATG CACAAGGGCG CCATCCATAT GCCTACGTGC 1441 CCTTCTCTGC TGGCCCCAGG AACTGTATAG GTCAAAAGTT TGCTGTGATG GAAGAAAAGA 1501 CCATTCTTTC GTGCATCCTG AGGCACTTTT GGATAGAATC CAACCAGAAA AGAGAAGAGC 1561 TTGGTCTAGA AGGACAGTTG ATTCTTCGTC CAAGTAATGG CATCTGGATC AAGTTGAAGA 1621 GGAGAAATGC AGATGAACGC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1681 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1741 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGAA TCTGCACGCA 1801 GGCCGTTCTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt