Construct: ORF TRCN0000478676
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014999.1_s317c1
- Derived from:
- ccsbBroadEn_08403
- DNA Barcode:
- ATGGCCGCGACGAATACATGTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MANSC1 (54682)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478676
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54682 | MANSC1 | MANSC domain containing 1 | NM_018050.4 | 99.8% | 99.7% | 163G>A;1173C>T |
| 2 | human | 54682 | MANSC1 | MANSC domain containing 1 | NM_001363613.2 | 91.9% | 91.8% | 38_39ins102;61G>A;1071C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1359
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cttcggggga gaagggagct tgacttacac tttggtaata atttgcttcc 121 tgacactaag gctgtctgct agtcagaatt gcctcaaaaa gagtctagaa gatgttgtca 181 ttgacatcca gtcatctctt tctaagggaa tcagaggcaa tgagcccata tatacttcaa 241 ctcaagaaga ctgcattaat tcttgctgtt caacaaaaaa catatcaggg gacaaagcat 301 gtaacttgat gatcttcgac actcgaaaaa cagctagaca acccaactgc tacctatttt 361 tctgtcccaa cgaggaagcc tgtccattga aaccagcaaa aggacttatg agttacagga 421 taattacaga ttttccatct ttgaccagaa atttgccaag ccaagagtta ccccaggaag 481 attctctctt acatggccaa ttttcacaag cagtcactcc cctagcccat catcacacag 541 attattcaaa gcccaccgat atctcatgga gagacacact ttctcagaag tttggatcct 601 cagatcactt ggagaaacta tttaagatgg atgaagcaag tgcccagctc cttgcttata 661 aggaaaaagg ccattctcag agttcacaat tttcctctga tcaagaaata gctcatctgc 721 tgcctgaaaa tgtgagtgcg ctcccagcta cggtggcagt tgcttctcca cataccacct 781 cggctactcc aaagcccgcc acccttctac ccaccaatgc ttcagtgaca ccttctggga 841 cttcccagcc acagctggcc accacagctc cacctgtaac cactgtcact tctcagcctc 901 ccacgaccct catttctaca gtttttacac gggctgcggc tacactccaa gcaatggcta 961 caacagcagt tctgactacc acctttcagg cacctacgga ctcgaaaggc agcttagaaa 1021 ccataccgtt tacagaaatc tccaacctaa ctttGAACAC AGGGAATGTG TATAACCCTA 1081 CTGCACTTTC TATGTCAAAT GTGGAGTCTT CCACTATGAA TAAAACTGCT TCCTGGGAAG 1141 GTAGGGAGGC CAGTCCAGGC AGTTCCTCCC AGGGCAGTGT TCCAGAAAAT CAGTACGGCC 1201 TTCCATTTGA AAAATGGCTT CTTATCGGGT CCCTGCTTTT TGGTGTCCTG TTCCTGGTGA 1261 TAGGCCTCGT CCTCCTGGGT AGAATCCTCT CGGAATCACT CCGCAGGAAA CGTTACTCAA 1321 GACTGGATTA TTTGATCAAT GGGATCTATG TGGACATCTA CCCAACTTTC TTGTACAAAG 1381 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1441 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1501 ACGAATGGCC GCGACGAATA CATGTTGTAC GCGTTAAGTC gacaatcaac ctctggatta 1561 caaaatttgt gaaagatt