Transcript: Human NM_001363613.2

Homo sapiens MANSC domain containing 1 (MANSC1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
MANSC1 (54682)
Length:
5430
CDS:
266..1459

Additional Resources:

NCBI RefSeq record:
NM_001363613.2
NBCI Gene record:
MANSC1 (54682)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001363613.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000242952 GAGGCAATGAGCCCGTATATA pLKO_005 312 CDS 100% 15.000 21.000 N MANSC1 n/a
2 TRCN0000242951 CAACCCAACTGCTACCTATTT pLKO_005 437 CDS 100% 13.200 18.480 N MANSC1 n/a
3 TRCN0000257017 GTTACTCAAGACTGGATTATT pLKO_005 1410 CDS 100% 15.000 10.500 N MANSC1 n/a
4 TRCN0000148618 CGTTACTCAAGACTGGATTAT pLKO.1 1409 CDS 100% 13.200 9.240 N MANSC1 n/a
5 TRCN0000242950 GTCTTAGCAGGAGGTTGTATT pLKO_005 1534 3UTR 100% 13.200 9.240 N MANSC1 n/a
6 TRCN0000242953 GAAATCTCCAACCTAACTTTG pLKO_005 1133 CDS 100% 10.800 7.560 N MANSC1 n/a
7 TRCN0000149843 CAGGAAGATTCTCTCTTACAT pLKO.1 572 CDS 100% 5.625 3.938 N MANSC1 n/a
8 TRCN0000178780 CGACCCTCATTTCTACAGTTT pLKO.1 1002 CDS 100% 4.950 3.465 N MANSC1 n/a
9 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2950 3UTR 100% 4.950 2.475 Y ERAP2 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2951 3UTR 100% 13.200 6.600 Y LIAS n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1840 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 2605 3UTR 100% 2.640 1.320 Y LINC01098 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1840 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001363613.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08403 pDONR223 100% 91.9% 91.8% None 38_39ins102;61G>A;1071C>T n/a
2 ccsbBroad304_08403 pLX_304 0% 91.9% 91.8% V5 38_39ins102;61G>A;1071C>T n/a
3 TRCN0000478676 ATGGCCGCGACGAATACATGTTGT pLX_317 23.9% 91.9% 91.8% V5 38_39ins102;61G>A;1071C>T n/a
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