Construct: ORF TRCN0000478696
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017942.2_s317c1
- Derived from:
- ccsbBroadEn_11034
- DNA Barcode:
- TACGTCGACAGGACTCTGAACAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLSCR1 (5359)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478696
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | NM_021105.3 | 37.3% | 33.6% | (many diffs) |
| 2 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | XM_011512904.1 | 37.3% | 33.6% | (many diffs) |
| 3 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | XM_017006629.1 | 37.3% | 33.6% | (many diffs) |
| 4 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | XM_017006628.1 | 37.2% | 33.5% | (many diffs) |
| 5 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | NM_001363872.1 | 35.1% | 31.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga caaacaaaac tcacagatga atgcttctca cccggaaaca aacttgccag 121 ttgggtatcc TCCTCAGTAT CCACCGACAG CATTCCAAGG ACCTCCAGGA TATAGTGGCT 181 ACCCTGGGCC CCAGGTCAGC TACCCACCCC CACCAGCCGG CCATTCAGGT CCTGGCCCAG 241 CTGGCTTTCC TGTCCCAAAT CAGCCAGTGT ATAATCAGCC AGTATATAAT CAGCCAGTTG 301 GAGCTGCAGG GGTACCATGG ATGCCAGCGC CACAGCCTCC ATTAAACTGT CCACCTGGAT 361 TAGAATATTT AAGTCAGGTA ATTTCAAAGA CACAAAATAC TCATAAAAAA CAGAACTGTG 421 CTTCCAGCTT GCTTAACCAG ATTAGCAAAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATACGT 601 CGACAGGACT CTGAACAAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt