Construct: ORF TRCN0000478703
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003870.1_s317c1
- Derived from:
- ccsbBroadEn_04915
- DNA Barcode:
- TGTCCTCGAACCGTAACATCCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9orf116 (138162)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478703
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 138162 | C9orf116 | chromosome 9 open reading f... | NM_144654.3 | 100% | 100% | |
| 2 | human | 138162 | C9orf116 | chromosome 9 open reading f... | NM_001048265.2 | 67.6% | 55.7% | 223_268del;323_408del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 366
- ORF length:
- 276
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCTGAGGA ATGCCCCAGA GCGTGCGCGG 121 AGCCTGTGGC GCCCAAGGCC ACGGCCCCGC CGGAGAGGAC CAGCGACTAC TACCGCGTGA 181 GCGCGGACCT GCCGGGCAGG TTCAACAACC CGGGGTGGTT CCGGGGCTAC AGGACCCAGA 241 AGGCTGTCTC CGTGTACAGG ACCAGTAACC AGGCTTACGG GAGCAGAGCC CCCACCGTGC 301 ACGAGATGCC TCGGGTGGAA TGTTCCGGAA CAATACTCTC AATGTTTACC TGGAGAAAAG 361 CATCGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 421 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 481 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGTCCTCGA ACCGTAACAT CCGTCACGCG 541 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt