Construct: ORF TRCN0000478706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014455.2_s317c1
- Derived from:
- ccsbBroadEn_15058
- DNA Barcode:
- CGGTCATACCTACTTGGAGCCCTC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDON (50937)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478706
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50937 | CDON | cell adhesion associated, o... | NM_016952.4 | 89.3% | 89.3% | (many diffs) |
| 2 | human | 50937 | CDON | cell adhesion associated, o... | XM_011542866.3 | 89.3% | 89.3% | (many diffs) |
| 3 | human | 50937 | CDON | cell adhesion associated, o... | NM_001243597.1 | 87.7% | 87.7% | (many diffs) |
| 4 | human | 50937 | CDON | cell adhesion associated, o... | XM_011542862.3 | 87.7% | 87.7% | (many diffs) |
| 5 | human | 50937 | CDON | cell adhesion associated, o... | XM_011542863.2 | 87.7% | 87.7% | (many diffs) |
| 6 | human | 50937 | CDON | cell adhesion associated, o... | XM_011542864.2 | 87.7% | 87.7% | (many diffs) |
| 7 | human | 50937 | CDON | cell adhesion associated, o... | XM_011542865.2 | 87.7% | 87.7% | (many diffs) |
| 8 | human | 50937 | CDON | cell adhesion associated, o... | XM_017017873.1 | 87.7% | 87.7% | (many diffs) |
| 9 | human | 50937 | CDON | cell adhesion associated, o... | XR_001747899.2 | 41.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3474
- ORF length:
- 3405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcatccggat cttggaccct tatgtacact gctgtatgtt actcttacaa 121 ttctgtgctc ttctgtgagt tcagacttgg caccttattt tacttctgag ccgctctctg 181 ctgtccagaa acttggtgga cctgtagtac tgcattgttc tgctcaacct gtgaccactc 241 gtatctcatg gctgcataac ggaaaaacat tggatggaaa cctggaacat attaagattc 301 atcaggggac tctgacaatt ctttctctca actcctctct tttgggttac taccagtgcc 361 ttgccaacaa tagcatcggt gccattgtga gtggccctgc gacagtatct gtggcagttc 421 ttggtgattt tggttcatcc acaaagcatg ttattacagc agaagaaaaa agtgctggtt 481 tcattggctg cagggtaccg gagagtaacc ccaaagctga ggtgcgctat aaaatccggg 541 gaaaatggct gaaacattcc acagagaatt acttaatcct tccatcagga aatcttcaga 601 ttttgaatgt atccttagag gacaagggat catacaaatg tgcagcttat aatcctgtca 661 cacatcaatt aaaagttgaa cctattggcc gaaagctcct tgtgagtcgt ccttcttcag 721 atgatgttca cattcttcac cccacccatt cacaggcatt agctgttctt tctcgtagcc 781 ctgtaacctt ggagtgtgtg gtgagtgggg tcccggctcc tcaagtgtat tggctaaagg 841 acgggcagga cattgcacca ggaagcaact ggagaaggtt gtattctcat cttgccactg 901 atagcgttga cccggcggac tccggaaact attcctgcat ggcgggaaac aagtctggag 961 atgtaaaata tgtgacttac atggttaatg tacttgaaca tgcttccatt tctaaaggac 1021 tacaggatca gatagtgtct ctgggtgcca cagtacactt tacctgcgac gttcatggga 1081 acccagcccc caactgtacc tggtttcaca atgcacagcc tattcatcct tctgcacgac 1141 atctaactgc aggaaacgga ctgaaaatca gtggggttac tgtggaagat gttgggatgt 1201 atcagtgtgt agcagataat gggattggat ttatgcactc tactggaaga cttgaaattg 1261 aaaatgacgg tggattcaag ccagttataa ttacggcacc agtaagtgca aaggttgcag 1321 acggagactt tgttactctg tcctgcaatg ccagtgggct gccggttccg gtcattcgtt 1381 ggtatgacag ccatggattg ataaccagcc atccatctca agtcctgaga tcgaaatccc 1441 gaaaatcaca gttatcaaga cctgagggct tgaacctgga gcctgtgtac ttcgtcctgt 1501 cccaagctgg tgcaagctct ctccatattc aggctgtgac tcaggaacat gcggggaaat 1561 acatctgcga agctgcaaat gaacatggta ccacacaggc agaagcatct ctcatggttg 1621 ttccttttga aacaaataca aaagcagaga cagtcacact tcctgatgct gctcagaatg 1681 atgacagaag taagagagat ggttcagaaa ctgggttact gagctcattt ccggtgaagg 1741 tccatcccag tgcagtggaa tcagcaccag agaaaaacgc cagcggcatc tctgttcctg 1801 atgcccccat catactgagc cccccacaga cccacacacc agacacgtac aacctggtgt 1861 ggagggcagg caaggatggt gggctgccca tcaatgctta ctttgtgaag tatcgaaagc 1921 tggatgatgg ggttggcatg ctgggaagct ggcacacggt tcgagtccca ggaagtgaaa 1981 atgagctcca tttagctgag ctggagccat ctagtcttta tgaagtcttg atggtagcaa 2041 gaagcgcagc aggtgaaggc caacctgcca tgcttacctt ccgaaccagc aaagaaaaaa 2101 cagcgtcatc aaaaaacacc caggcatcct ctccacccgt gggcatccct aagtatcccg 2161 ttgtttcaga ggctgcaaac aacaattttg gagtggtact tacagattcc tctaggcaca 2221 gtggagttcc agaggcacca gatcggccta ccatctccac tgcatcagag acatcagtct 2281 atgtcacttg gattcctcgg gcaaacgggg gttctccaat cactgccttc aaagtcgaat 2341 ataaacggat gaggaccagc aattggctgg tggcagctga agacatccct ccttccaaac 2401 tttcagtgga agttcgtagt ttagaaccag gttcaacata caaatttagg gtcattgcca 2461 tcaaccatta tggtgagagt tttcggagtt cagcatctcg tccttatcaa gtggttgggt 2521 tccccaatcg cttttccagc cgtccaataa ctggacctca cattgcatac acagaggctg 2581 tcagcgatac tcagatcatg ctaaagtgga cgtacattcc atcaagtaac aataacactc 2641 ccattcaagg attttatatc tattaccgac caacagatag tgacaatgac agtgattaca 2701 agagggatgt tgtagaaggt tcaaagcagt ggcacatgat tggccacctg cagccagaaa 2761 cctcctatga cattaaaatg caatgcttca atgaaggagg agaaagtgaa tttagcaatg 2821 tgatgatctg cgagactaaa gtgaaacgtg ttcctggagc ttctgaatat cctgtcaaag 2881 acttgagtac ccctccaaat tctttgggaa gtggaggaaa tgtggggcct gcaaccagcc 2941 ctgccagaag cagtgacatg ttatatctga tcgttggctg tgtgctgggc gtcatggtcc 3001 tcattctgat ggttttcatt gcaatgtgcc tgtggaagaa tcgccagcag aataccatac 3061 aaaaatatga cccaccagga tatctctacc aaggatcaga tatgaacggg cagatggtgg 3121 actacaccac tctcTCAGGA GCAAGTCAGA TAAATGGAAA TGTTCACGGA GGCTTCCTAA 3181 CCAATGGCGG TCTCAGCAGT GGCTATTCCC ACCTTCACCA TAAGGTCCCC AATGCAGTCA 3241 ATGGAATTGT GAATGGGAGC CTAAATGGAG GGCTTTACTC CGGGCACAGC AACTCTCTAA 3301 CCAGGACACA CGTGGATTTT GAACATCCTC ATCATCTAGT GAATGGTGGT GGAATGTACA 3361 CGGCCGTGCC TCAGATTGAC CCTCTGGAGT GTGTTAACTG CCGAAATTGT CGAAACAACA 3421 ATAGGTGTTT CACCAAAACC AACAGCACTT TCAGCAGCAG CCCCGGGAAA CCTTGCCAAC 3481 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 3541 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 3601 ATCTTGTGGA AAGGACGACG GTCATACCTA CTTGGAGCCC TCACGCGTTA AGTCgacaat 3661 caacctctgg attacaaaat ttgtgaaaga tt