Construct: ORF TRCN0000478716
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001389.1_s317c1
- Derived from:
- ccsbBroadEn_08570
- DNA Barcode:
- AGCCCGAAATTGCTGTACTGAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSR1 (55720)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478716
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55720 | TSR1 | TSR1 ribosome maturation fa... | NM_018128.5 | 99.9% | 100% | 1419G>A |
| 2 | mouse | 104662 | Tsr1 | TSR1 20S rRNA accumulation | NM_177325.3 | 84.4% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2481
- ORF length:
- 2412
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcccac cgccccggcc cgctcaagca gcagaataaa gctcataaag 121 gcggacggca tcggggtcgg ggatctgcac agcgggacgg caagggccgt ctggcactga 181 aaaccctaag caagaaggtg agaaaagaac tcagcagagt cgaccagagg catcgcgcca 241 gccagctccg aaagcagaag aaggaggcgg ttctggcaga gaagagacag ctgggtggca 301 aggatggccc tcctcatcag gtactggtgg tgcccctgca cagcagaatt tccctgccag 361 aggccatgca gctgcttcaa gatagggaca ctggaacagt acacttgaat gaattgggaa 421 acacccagaa ctttatgctg ctgtgccccc gcttgaaaca tcggtggttt ttcacctcag 481 caaggccagg ggatctgcac gttgtgttag acatggctaa agtagctgat accatcctgt 541 tcctccttga tccactagaa ggctgggaca gcaccggtga ttactgtctt tcctgcctct 601 ttgctcaggg ccttccgacc tatacactag ctgtccaggg gatttctggc ctcccactga 661 agaaacaaat agataccagg aagaagctaa gtaaagcagt ggagaagcgc tttccgcatg 721 acaaactcct cttgttagac actcaacagg aggcagggat gctgcttagg cagttggcta 781 accagaagca acagcatctt gcttttcgag atcggcgggc ctacctattt gcccatgctg 841 ttgattttgt tcctagtgaa gagaataact tggtgggcac cttgaaaatt tcaggctatg 901 ttcgagggca gactctgaat gtcaataggt tgctgcatat cgttggatat ggtgatttcc 961 agatgaaaca gatagatgcc cccggagacc ctttcccttt aaatcctaga ggaattaaac 1021 cccaaaagga cccagacatg gcaatggaga tttgtgctac ggatgctgta gatgatatgg 1081 aagaaggtct taaagtccta atgaaggcag accctggtag acaggaatcc ttgcaagcag 1141 aggttatccc agatccaatg gagggagagc aaacctggcc cactgaggag gagctgagcg 1201 aggcaaagga tttcttgaag gaaagttcta aggtggtaaa gaaggtcccc aaaggaacat 1261 ccagttacca agctgaatgg attttggatg gtggcagcca aagtggtggg gaaggagatg 1321 aatatgaata tgatgatatg gaacatgagg attttatgga ggaggaatct caggatgaga 1381 gtagtgaaga agaggaagaa tatgaaacta tgactattgg ggagtctgtg catgatgatc 1441 tgtatgataa gaaagtagat gaagaagctg aggcaaaaat gttggaaaaa tataaacaag 1501 aaagactgga agagatgttt ccagatgaag tggacacgcc ccgtgatgtg gctgctcgaa 1561 ttcgatttca gaaatacaga ggccttaaga gcttccggac atctccatgg gatcctaagg 1621 aaaaccttcc tcaagattat gctcgaatat ttcagtttca gaactttact aacactagga 1681 aaagcatctt taaagaggtt gaagaaaaag aggttgaagg agctgaggtt ggctggtatg 1741 tcacacttca tgtctctgaa gtccccgtct cagtggtcga gtgcttcagg caaggaacac 1801 ccttgattgc attttcttta ctacctcatg aacagaagat gtcagtattg aatatggtgg 1861 tgaggcgtga ccctggcaac actgaacctg tgaaagccaa ggaagagctc atatttcact 1921 gtggattcag gcgcttccga gcctcacctt tattctctca gcacactgca gcggacaaac 1981 ataaattgca gagattcctg actgctgaca tggccctggt ggcgacagtc tatgcgccaa 2041 tcacttttcc tcctgcatct gtgctgcttt tcaagcaaaa aagcaatgga atgcacagcc 2101 TCATTGCTAC AGGCCATCTT ATGTCAGTAG ATCCAGACAG AATGGTCATC AAGAGAGTTG 2161 TTCTGAGTGG TCATCCTTTC AAAATTTTTA CTAAGATGGC AGTAGTACGT TACATGTTCT 2221 TCAACAGAGA GGATGTGCTG TGGTTTAAAC CAGTGGAACT GAGAACGAAG TGGGGCCGGA 2281 GAGGACATAT CAAGGAACCT TTAGGTACCC ATGGCCACAT GAAATGCAGC TTTGATGGGA 2341 AGCTAAAATC TCAAGACACA GTACTGATGA ACCTGTATAA ACGAGTCTTC CCCAAATGGA 2401 CTTATGATCC ATATGTACCA GAACCAGTAC CCTGGCTGAA AAGTGAGATT TCTTCAACAG 2461 TGCCTCAAGG GGGCATGGAG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2521 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2581 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTTGGAAAG GACGAAGCCC GAAATTGCTG 2641 TACTGAGGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt