Construct: ORF TRCN0000478751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011298.2_s317c1
- Derived from:
- ccsbBroadEn_03081
- DNA Barcode:
- GCCATCTCTTGCTCGCTAACCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NXT1 (29107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478751
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29107 | NXT1 | nuclear transport factor 2 ... | NM_013248.3 | 100% | 100% | |
| 2 | mouse | 56488 | Nxt1 | NTF2-related export protein 1 | NM_001110159.1 | 91.4% | 98.5% | (many diffs) |
| 3 | mouse | 56488 | Nxt1 | NTF2-related export protein 1 | NM_019761.6 | 91.4% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGGC ATCTGTGGAT TTCAAGACCT ATGTGGATCA GGCCTGCAGA GCTGCTGAGG 121 AGTTTGTCAA TGTCTACTAC ACCACCATGG ATAAGCGGCG GCGTTTGCTG TCCCGCCTGT 181 ACATGGGCAC AGCCACCCTG GTCTGGAATG GCAATGCTGT TTCAGGACAA GAATCCTTGA 241 GTGAGTTTTT TGAAATGTTG CCTTCCAGCG AGTTCCAAAT CAGCGTGGTA GACTGCCAGC 301 CTGTTCATGA TGAAGCCACA CCAAGCCAGA CCACGGTCCT TGTTGTCATC TGTGGATCAG 361 TGAAGTTTGA GGGGAACAAA CAACGGGACT TCAACCAGAA CTTCATCCTG ACCGCCCAGG 421 CCTCACCCAG CAACACAGTG TGGAAGATCG CAAGTGACTG CTTCCGCTTC CAGGACTGGG 481 CCAGCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCATCTCT TGCTCGCTAA CCGCAACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt