Construct: ORF TRCN0000478773
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014206.1_s317c1
- Derived from:
- ccsbBroadEn_03498
- DNA Barcode:
- ATCGAATATGCTTTACAGGTTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GEMIN8 (54960)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478773
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | NM_001042479.2 | 100% | 100% | |
| 2 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | NM_001042480.2 | 100% | 100% | |
| 3 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | NM_017856.3 | 100% | 100% | |
| 4 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XM_017029617.2 | 100% | 100% | |
| 5 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XM_017029618.2 | 100% | 100% | |
| 6 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XM_005274555.3 | 70.5% | 70.5% | 1_303del |
| 7 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_001755705.2 | 43.1% | 1_173del;900_1681del | |
| 8 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_001755704.2 | 42.5% | 1_173del;900_1708del | |
| 9 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_001755702.1 | 40.5% | 1_257del;984_1792del | |
| 10 | human | 492303 | GEMIN8P4 | gem nuclear organelle assoc... | NR_002830.1 | 39.2% | (many diffs) | |
| 11 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_001755703.1 | 35.3% | 1_257del;984_2054del | |
| 12 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_950466.3 | 13.7% | 1_3811del;4538_5299del | |
| 13 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_950464.3 | 13.5% | 1_3811del;4538_5348del | |
| 14 | human | 54960 | GEMIN8 | gem nuclear organelle assoc... | XR_950467.3 | 12.9% | 1_3811del;4538_5608del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 792
- ORF length:
- 726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcggtaaag gcatcaacat cgaaagctac caggccttgg tattctcatc 121 cggtatatgc aagatactgg caacattatc atcaagcaat ggcttggatg caaagccatc 181 acaatgccta caggaaggcc gtggaatcct gtttcaatct tccatggtac ttaccttctg 241 cgcttcttcc ccaaagctct tacgataatg aggctgcgta tcctcagtcc ttctatgacc 301 atcatgtggc ctggcaggac tacccctgca gttcttcaca tttcagaaga tctgggcagc 361 atccacgtta cagcagtagg atccaggcat ccacaaaaga agaccaagct ttgtccaaag 421 aggaagagat ggagactgag TCAGATGCAG AGGTAGAATG TGACCTGAGC AATATGGAAA 481 TCACTGAAGA GCTCCGCCAG TACTTTGCAG AGACCGAGAG GCATAGAGAA GAACGACGGC 541 GGCAGCAGCA GCTGGATGCA GAGCGCCTGG ACAGCTATGT GAACGCTGAC CACGACCTGT 601 ACTGCAACAC CCGCCGGTCG GTAGAAGCCC CAACTGAGAG GCCTGGTGAG CGGCGCCAGG 661 CCGAGATGAA GCGTTTGTAC GGGGACAGTG CTGCCAAGAT CCAAGCCATG GAGGCCGCGG 721 TGCAGCTGAG CTTTGACAAG CACTGTGACC GAAAGCAGCC CAAGTACTGG CCGGTCATCC 781 CCCTGAAGTT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATC GAATATGCTT TACAGGTTTA 961 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t