Construct: ORF TRCN0000478780
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007665.1_s317c1
- Derived from:
- ccsbBroadEn_02117
- DNA Barcode:
- TTGCACAACGTAAGCTGCACAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNMA1 (9240)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478780
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9240 | PNMA1 | PNMA family member 1 | NM_006029.5 | 100% | 100% | |
| 2 | mouse | 70481 | Pnma1 | paraneoplastic antigen MA1 | NM_027438.3 | 87.3% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1125
- ORF length:
- 1059
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gatgacactg ttggaagact ggtgccgggg gatggatgtg aactcccaga 121 gagctctgtt agtctggggc atcccagtga actgtgatga ggctgaaatc gaagagaccc 181 tccaggctgc gatgccccag gtctcctacc gaatgcttgg gagaatgttc tggagggaag 241 aaaatgcgaa agcagcctta ttagagctca ctggcgctgt agattacgcc gcgatcccca 301 gggagatgcc gggcaaagga ggggtctgga aagtgttatt taagccccca acttctgatg 361 ctgaattttt agaaagattg cacctcttcc tagctagaga ggggtggacc gtgcaagatg 421 ttgcccgtgt ccttgggttt cagaacccta ctccgacccc gggcccagag atgccagcag 481 agatgctaaa ctatattttg gataatgtta ttcagcctct tgttgagtcc atatggtaca 541 agaggctgac acttttctcg gggagggaca tcccagggcc tggagaggaa acctttgatc 601 cctggctgga gcacactaat gaggtcctag aggagtggca ggtgtccgat gtagaaaaga 661 ggcggcggtt gatggagagt cTTAGAGGCC CCGCCGCTGA TGTTATTCGC ATCCTTAAGT 721 CCAACAACCC CGCGATAACC ACTGCCGAAT GCCTGAAGGC GCTTGAGCAG GTGTTTGGGA 781 GCGTTGAGAG CTCTAGGGAT GCCCAGATCA AATTTCTGAA CACTTATCAG AACCCGGGAG 841 AAAAATTGTC TGCTTATGTC ATTCGTCTGG AGCCTCTGCT ACAGAAGGTG GTAGAGAAGG 901 GGGCCATTGA TAAAGATAAT GTGAACCAGG CCCGCCTAGA GCAGGTCATT GCCGGGGCCA 961 ACCACAGCGG GGCCATCCGA AGGCAGCTGT GGCTTACCGG GGCTGGGGAA GGGCCAGCCC 1021 CAAACCTCTT TCAGTTGCTG GTGCAGATCC GTGAGGAGGA AGCCAAGGAG GAGGAGGAGG 1081 AGGCTGAGGC CACCCTTCTG CAGTTAGGCC TGGAAGGGCA CTTCTGCCCA ACTTTCTTGT 1141 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1201 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1261 GAAAGGACGA TTGCACAACG TAAGCTGCAC AGGTACGCGT TAAGTCgaca atcaacctct 1321 ggattacaaa atttgtgaaa gatt