Construct: ORF TRCN0000478792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013479.1_s317c1
- Derived from:
- ccsbBroadEn_00461
- DNA Barcode:
- GTTGCCATCAACACATTCGCATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPYSL2 (1808)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1808 | DPYSL2 | dihydropyrimidinase like 2 | NM_001386.6 | 99.9% | 100% | 426T>C |
| 2 | human | 1808 | DPYSL2 | dihydropyrimidinase like 2 | NM_001244604.2 | 93.6% | 93.7% | 0_1ins108;318T>C |
| 3 | human | 1808 | DPYSL2 | dihydropyrimidinase like 2 | NM_001197293.3 | 83.8% | 83% | (many diffs) |
| 4 | mouse | 12934 | Dpysl2 | dihydropyrimidinase-like 2 | NM_009955.3 | 91.4% | 98.7% | (many diffs) |
| 5 | mouse | 12934 | Dpysl2 | dihydropyrimidinase-like 2 | XM_006518508.3 | 76.9% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1782
- ORF length:
- 1716
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttatcagggg aagaaaaata ttccacgcat cacgagcgat cgtcttctga 121 tcaaaggagg taaaattgtt aatgatgacc agtcgttcta tgcagacata tacatggaag 181 atgggttgat caagcaaata ggagaaaatc tgattgtgcc aggaggagtg aagaccatcg 241 aggcccactc ccggatggtg atccccggag gaattgacgt ccacactcgt ttccagatgc 301 ctgatcaggg aatgacgtct gctgatgatt tcttccaagg aaccaaggcg gccctggctg 361 ggggaaccac tatgatcatt gaccacgttg ttcctgagcc tgggacaagc ctgctcgctg 421 cctttgacca gtggagggaa tgggccgaca gcaagtcctg ctgtgactac tctctgcatg 481 tggacatcag cgagtggcat aagggcatcc aggaggagat ggaagcgctt gtgaaggatc 541 acggggtaaa ttccttcctc gtgtacatgg ctttcaaaga tcgcttccag ctaacggatt 601 gccagattta tgaagtactg agtgtgatcc gggatattgg cgccatagcc caagtccacg 661 cagaaaatgg cgacatcatt gcagaggagc agcagaggat cctggatctg ggcatcacgg 721 gccccgaggg acatgtgctg agccgacctg aggaggtcga ggccgaagcc gtgaatcgtg 781 ccatcaccat cgccaaccag accaactgcc cgctgtatat caccaaggtg atgagcaaaa 841 gctctgctga ggtcatcgcc caggcacgga agaagggaac tgtggtgtat ggcgagccca 901 tcactgccag cttgggaacg gacggctccc attactggag caagaactgg gccaaggctg 961 ctgcctttgt cacctcccca cccttgagcc ctgatccaac cactccagac tttctcaact 1021 ccttgctgtc ctgtggagac ctccaggtca cgggcagtgc ccattgcacg tttaacactg 1081 cccagaaggc tgtaggaaag gacaacttca ccctgattcc ggagggcacc aatggcactg 1141 aggagcggat gtccgtcatc tgggacaagg ctgtggtcac tgggaagatg gatgagaacc 1201 agtttgtggc tgtgaccagc accaatgcag ccaaagtctt caacctttac ccccggaaag 1261 gccgcattgc tgtgggatcc gatgccgacc tggtcatctg ggaccccgac agcgttaaaa 1321 ccatctctgc caagacacac aacagctctc tcgagtacaa catctttgaa ggcatggagt 1381 gccgcggctc cccactggtg gtcatcagcc aggggaagat tgtcctggag gacggcaccc 1441 TGCATGTCAC CGAAGGCTCT GGACGCTACA TTCCCCGGAA GCCCTTCCCT GATTTTGTTT 1501 ACAAGCGTAT CAAGGCAAGG AGCAGGCTGG CTGAGCTGAG AGGGGTTCCT CGTGGCCTGT 1561 ATGACGGACC TGTGTGTGAA GTGTCTGTGA CGCCCAAGAC AGTCACTCCA GCCTCCTCGG 1621 CCAAGACGTC TCCTGCCAAG CAGCAGGCCC CACCTGTCCG GAACCTGCAC CAGTCTGGAT 1681 TCAGTTTGTC TGGTGCTCAG ATTGATGACA ACATTCCCCG CCGCACCACC CAGCGTATCG 1741 TGGCGCCCCC CGGTGGCCGT GCCAACATCA CCAGCCTGGG CTACCCAACT TTCTTGTACA 1801 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1861 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1921 AGGACGAGTT GCCATCAACA CATTCGCATT TACGCGTTAA GTCgacaatc aacctctgga 1981 ttacaaaatt tgtgaaagat t