Construct: ORF TRCN0000478804
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017515.1_s317c1
- Derived from:
- ccsbBroadEn_13902
- DNA Barcode:
- AATCCAATAAAGGAAATGGTGGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MSN (4478)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478804
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4478 | MSN | moesin | NM_002444.3 | 99.9% | 99.4% | 1724_1725insG |
| 2 | human | 4478 | MSN | moesin | XM_005262269.2 | 99.4% | 98.6% | 3_4insCC;10_15delAACCAAinsG;1727_1728insG |
| 3 | human | 4478 | MSN | moesin | XM_017029545.1 | 98% | 97.5% | 0_1ins33;1691_1692insG |
| 4 | human | 4478 | MSN | moesin | XM_017029546.1 | 98% | 97.5% | 0_1ins33;1691_1692insG |
| 5 | human | 4478 | MSN | moesin | XM_011530959.1 | 94.3% | 93.4% | (many diffs) |
| 6 | mouse | 17698 | Msn | moesin | NM_010833.2 | 92% | 98% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1800
- ORF length:
- 1734
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caaaacgatc agtgtgcgtg tgaccaccat ggatgcagag ctggagtttg 121 ccatccagcc caacaccacc gggaagcagc tatttgacca ggtggtgaaa actattggct 181 tgagggaagt ttggttcttt ggtctgcagt accaggacac taaaggtttc tccacctggc 241 tgaaactcaa taagaaggtg actgcccagg atgtgcggaa ggaaagcccc ctgctcttta 301 agttccgtgc caagttctac cctgaggatg tgtccgagga attgattcag gacatcactc 361 agcgcctgtt ctttctgcaa gtgaaagagg gcattctcaa tgatgatatt tactgcccgc 421 ctgagaccgc tgtgctgctg gcctcgtatg ctgtccagtc taagtatggc gacttcaata 481 aggaagtgca taagtctggc tacctggccg gagacaagtt gctcccgcag agagtcctgg 541 aacagcacaa actcaacaag gaccagtggg aggagcggat ccaggtgtgg catgaggaac 601 accgtggcat gctcagggag gatgctgtcc tggaatatct gaagattgct caagatctgg 661 agatgtatgg tgtgaactac ttcagcatca agaacaagaa aggctcagag ctgtggctgg 721 gggtggatgc cctgggtctc aacatctatg agcagaatga cagactaact cccaagatag 781 gcttcccctg gagtgaaatc aggaacatct ctttcaatga taagaaattt gtcatcaagc 841 ccattgacaa aaaagccccg gacttcgtct tctatgctcc ccggctgcgg attaacaagc 901 ggatcttggc cttgtgcatg gggaaccatg aactatacat gcgccgtcgc aagcctgata 961 ccattgaggt gcagcagatg aaggcacagg cccgggagga gaagcaccag aagcagatgg 1021 agcgtgctat gctggaaaat gagaagaaga agcgtgaaat ggcagagaag gagaaagaga 1081 agattgaacg ggagaaggag gagctgatgg agaggctgaa gcagatcgag gaacagacta 1141 agaaggctca gcaagaactg gaagaacaga cccgtagggc tctggaactt gagcaggaac 1201 ggaagcgtgc ccagagcgag gctgaaaagc tggccaagga gcgtcaagaa gctgaagagg 1261 ccaaggaggc cttgctgcag gcctcccggg accagaaaaa gactcaggaa cagctggcct 1321 tggaaatggc agagctgaca gctcgaaTCT CCCAGCTGGA GATGGCCCGA CAGAAGAAGG 1381 AGAGTGAGGC TGTGGAGTGG CAGCAGAAGG CCCAGATGGT ACAGGAAGAC TTGGAGAAGA 1441 CCCGTGCTGA GCTGAAGACT GCCATGAGTA CACCTCATGT GGCAGAGCCT GCTGAGAATG 1501 AGCAGGATGA GCAGGATGAG AATGGGGCAG AGGCTAGTGC TGACCTACGG GCTGATGCTA 1561 TGGCCAAGGA CCGCAGTGAG GAGGAACGTA CCACTGAGGC AGAGAAGAAT GAGCGTGTGC 1621 AGAAGCACCT GAAGGCCCTC ACTTCGGAGC TGGCCAATGC CAGAGATGAG TCCAAGAAGA 1681 CTGCCAATGA CATGATCCAT GCTGAGAACA TGCGACTGGG CCGAGACAAA TACAAGACCC 1741 TGCGCCAGAT CCGGCAGGGC AACACCAAGC AGCGCATTGA CGAATTTGAG GTCTATGTAC 1801 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 1861 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1921 TATATATCTT GTGGAAAGGA CGAAATCCAA TAAAGGAAAT GGTGGTAACG CGTTAAGTCg 1981 acaatcaacc tctggattac aaaatttgtg aaagatt