Construct: ORF TRCN0000478809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010927.1_s317c1
- Derived from:
- ccsbBroadEn_12074
- DNA Barcode:
- TTCCCTACAATATTCTCACAAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUTM2F (54754)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54754 | NUTM2F | NUT family member 2F | NM_017561.2 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 441457 | NUTM2G | NUT family member 2G | NM_001170741.2 | 97.2% | 96.9% | (many diffs) |
| 3 | human | 729262 | NUTM2B | NUT family member 2B | NM_001278495.1 | 78.7% | 72.1% | (many diffs) |
| 4 | human | 728118 | NUTM2A | NUT family member 2A | NM_001099338.2 | 78.7% | 71.8% | (many diffs) |
| 5 | human | 728118 | NUTM2A | NUT family member 2A | XM_011540122.2 | 78.7% | 71.8% | (many diffs) |
| 6 | human | 283008 | NUTM2E | NUT family member 2E | NM_001355263.1 | 78.4% | 71.7% | (many diffs) |
| 7 | human | 283008 | NUTM2E | NUT family member 2E | XM_024447950.1 | 78.4% | 71.7% | (many diffs) |
| 8 | human | 283008 | NUTM2E | NUT family member 2E | XM_024447951.1 | 78.4% | 71.7% | (many diffs) |
| 9 | human | 441457 | NUTM2G | NUT family member 2G | NM_001045477.3 | 64.2% | 63.1% | (many diffs) |
| 10 | human | 728130 | NUTM2D | NUT family member 2D | NM_001009610.1 | 58% | 55% | (many diffs) |
| 11 | human | 728118 | NUTM2A | NUT family member 2A | XM_017016607.1 | 53.4% | 50.5% | (many diffs) |
| 12 | human | 283008 | NUTM2E | NUT family member 2E | XM_024447952.1 | 53.3% | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2334
- ORF length:
- 2265
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttcaaat ggagcatacc cagtgctggg acccggcgtg accgtgaacc 121 ctggcacctc cctgtctgtg ttcacggctc tgccctttgc cacacccgct cccggcccag 181 cacacaggcc gcccctcgtg actgcagtgg ttcctccagc cggccctctg gtgctctctg 241 ccttccccag cacccctcta gtggcaggac aggatggccg cggcccgagt ggggccgggg 301 cttccaacgt ctttgtccag atgaggacag aagtggggcc tgtgaagccc cctcaggcac 361 agaccttgat cctaactcag gcccccctcg tctggcaggc tccaggcacc ctctgtggag 421 gtgtcatgtg tccacctccc ctattcctgg cagctgctcc tggggtgccc gttacctctg 481 cccaggtggt tgggggcacc caggcctgtg agggaggctg gtcccatggc cttcctcttc 541 caccaccacc accggctgcc caggtggccc ccatcgtgtc cccagggaac gctgggccat 601 ggccacaagg ggctcatgga gagggcagcc tggctccctc ccaggccaag gcccggccgg 661 acgactcctg taaacccaag agtgtctatg agaacttccg actctggcag cactacaagc 721 ccctggcccg gaggcacctt ccccagagtc ctgacactga agcgctttcc tgcttcctca 781 tcccagttct ccgatccctg gcccggcgga agcccaccat gacgctggag gagggactgt 841 ggcaggccat gcgggaatgg cagcacacga gcaactttga ccggatgatc ttctacgaga 901 tggcggaaaa gttcctggaa tttgaggctg aggaggagat gcagattcag aaatcgcagt 961 ggatgaaggg gccccagagc ctgcctcctc cagccccgcc gaggcttgaa cctcgaggac 1021 cccctgcccc tgaggtggtc aagcagccag tgtaccttcc cagcaaggat ggccccaagg 1081 ccccgactgc ctgcctgcca ccacccaggc cccagaggcc agcggagacc aaggcccacc 1141 tgccaccacc caggccccag aggccagcgg agaccaacgc ccacctgcca ccacccaggc 1201 cccagaggcc agcggagacc aaggtccctg aggagatccc ccctgaagtg gtgcaggagt 1261 atgtggacat catggaggag ctgctggggt ctcaccctgg ggacacaggg gagcctgagg 1321 gacaacggga aaagggcaaa gtggagcagc cgcaggaaga ggacgggata acctcagacc 1381 cgggcctcct gagctacatt gacaagctgt gttcccagga agactttgtc accaaggtgg 1441 aggccgtcat tcacccccga ttcctggaag aattgctttc cccagatcca cagatggatt 1501 tcttggccct aagccaggag ctggagcagg aggaaggact cacccttgcc cagctagtgg 1561 agaagcgcct cctgtccttg aaggagaaag ggtgcgggag ggcagcccct cgacatggca 1621 cggccaggtt ggactcaagt ccttctgagt ttgcagctgg ccaagaagca gcgagagagg 1681 tccctgaccc ccaacaaagg gtcagcgtgg aaacctcccc accccagacg gctgcccagg 1741 accctcaggg acagggcaga gtgcgcactg gcatggccag gtccgaagac cctgctgtgc 1801 ttttgggatg tcaggattcc cccaggctga aggctgtccg gccaacctct cctccccagg 1861 accacagacc cacctgcccc ggcctgggga ccaaggacgc cttgggtctc cctggagagt 1921 ctcctgtcaa ggagtcacat gggctggcta aggggtcaag tgaggagacg gaactccctg 1981 gcatggtcta tgtcgtgggt tcccaccaca ggctgaggcc ctggaggctg tcccagagcc 2041 ctgtcccttc ctcgggcctt cTCAGCCCAG GAGGGAGAGG ACCCCAGGGA GCTCTTCAGT 2101 CTCCATCTGC TCAGAAAAGA GGCCTCAGCC CATCACCTCC TGCCAGCAAG TCCAAGAAGC 2161 GACCTCTCTT TGGAAGCCCA TCCCCTGCTG AAAAGACACC GCACCCAGGG CCTGGGCTCA 2221 GGGTCTCTGG GGAGCAATCC CTGGCTTGGG GGCTGGGTGG CCCCTCACAG TCTCAAAAGA 2281 GAAAGGGTGA CCCCTTGGCC TCCAGGAGGA AGAAGAAGCG GCATTGTAGC CAGTTGCCAA 2341 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2401 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2461 TATCTTGTGG AAAGGACGAT TCCCTACAAT ATTCTCACAA GGCACGCGTT AAGTCgacaa 2521 tcaacctctg gattacaaaa tttgtgaaag att