Construct: ORF TRCN0000478824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012913.1_s317c1
- Derived from:
- ccsbBroadEn_02717
- DNA Barcode:
- GAGCCTGCCCGTACACACACTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EMC1 (23065)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478824
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23065 | EMC1 | ER membrane protein complex... | NM_015047.3 | 100% | 100% | |
| 2 | human | 23065 | EMC1 | ER membrane protein complex... | NM_001271427.2 | 99.8% | 99.8% | 1025_1026insGCA |
| 3 | human | 23065 | EMC1 | ER membrane protein complex... | NM_001271428.2 | 99.8% | 99.8% | 1308_1309insGCA |
| 4 | human | 23065 | EMC1 | ER membrane protein complex... | XM_005245787.2 | 99.6% | 99.6% | 1431_1439delAGCAATTCA |
| 5 | human | 23065 | EMC1 | ER membrane protein complex... | XM_005245788.2 | 99.5% | 99.5% | 1025_1026insGCA;1428_1436delAGCAATTCA |
| 6 | human | 23065 | EMC1 | ER membrane protein complex... | NM_001271429.2 | 97.7% | 97.6% | 220_221ins66 |
| 7 | mouse | 230866 | Emc1 | ER membrane protein complex... | NM_001039200.2 | 88.2% | 93.3% | (many diffs) |
| 8 | mouse | 230866 | Emc1 | ER membrane protein complex... | NM_146157.4 | 87.9% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3045
- ORF length:
- 2979
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctgagtgg gcttctcgtt tctggctttg ggctacgctg ctgattcctg 121 cggccgcggt ctacgaagac caagtgggca agtttgattg gagacagcaa tatgttggga 181 aggtcaagtt tgcctccttg gaattttccc ctggatccaa gaagttggtt gtagccacag 241 agaagaatgt gattgcagca ttaaattccc gaactgggga gatcttgtgg cgccatgttg 301 acaagggcac ggcagaaggg gctgtggatg ccatgctgct gcacggacag gatgtgatca 361 ctgtgtccaa tggaggccga atcatgcgtt cctgggagac taacatcggg ggcctgaact 421 gggagataac cctggacagt ggcagtttcc aggcacttgg gctggttggc ctgcaggagt 481 ctgtaaggta catcgcagtc ctgaagaaga ctacacttgc cctccatcac ctctccagtg 541 ggcacctcaa gtgggtggaa catctcccag aaagtgacag catccactac cagatggtgt 601 attcttacgg ctctggggtg gtgtgggccc tcggagttgt tcccttcagc catgtgaaca 661 ttgtcaagtt taatgtggaa gatggagaga ttgttcagca ggttagggtt tcaactccgt 721 ggctgcagca cctgtctgga gcctgtggtg tggtggatga ggctgtcctg gtgtgtcctg 781 acccgagctc acgttccctc caaactttgg ctctggagac ggaatgggag ttgagacaga 841 tcccactgca gtctctcgac ttagaatttg gaagtggatt ccaaccccgg gtcctgccta 901 cccagcccaa cccagtggac gcttcccggg cccagttctt cctgcacttg tccccaagcc 961 actatgctct gctgcagtac cattatggaa cgctgagttt gcttaaaaac ttcccacaga 1021 ctgccctagt gagctttgcc accactgggg agaagacggt ggctgcagtc atggcctgtc 1081 ggaatgaagt gcagaaaagt agcagttctg aagatgggtc aatggggagc ttttcggaga 1141 agtctagttc aaaggactct ctggcttgct tcaatcagac ctacaccatt aacctatacc 1201 tcgtggagac aggtcggcgg ctgctggaca ccacgataac atttagcctg gaacagagcg 1261 gcactcggcc tgagcggctg tatatccagg tgttcttgaa gaaggatgac tcagtgggct 1321 accgggcttt ggtgcagaca gaggatcatc tgctactttt cctgcagcag ttggcaggga 1381 aggtggtgct gtggagccgt gaggagtccc tggcagaagt ggtgtgccta gagatggtgg 1441 acctccccct gactggggca caggccgagc tggaaggaga atttggcaaa aaggcagatg 1501 gcttgctggg gatgttcctg aaacgcctct cgtctcagct tatcctgctg caagcatgga 1561 cttcccacct ctggaaaatg ttttatgatg ctcggaagcc ccggagtcag attaagaatg 1621 agatcaacat tgacaccctg gccagagatg aattcaacct ccagaagatg atggtgatgg 1681 taacagcctc aggcaagctt tttggcattg agagcagctc tggcaccatc ctgtggaaac 1741 agtatctacc caatgtcaag ccagactcct cctttaaact gatggtccag agaactactg 1801 ctcatttccc ccatccccca cagtgcaccc tgctggtgaa ggacaaggag tcgggaatga 1861 gttctctgta tgtcttcaat cccatttttg ggaagtggag tcaggtagct cccccagtgc 1921 tgaagcgccc catcttgcag tccttgcttc tcccagtcat ggatcaagac tacgccaagg 1981 tgttgctgtt gatagatgat gaatacaagg tcacagcttt tccagccact cggaatgtct 2041 tgcgacagct acatgagctt gccccttcca tcttcttcta tttggtggat gcagagcagg 2101 gacggctgtg tggatatcgg cttcgaaagg atctcaccac tgagctgagt tgggagctga 2161 ccattccccc agaagtacag cggatcgtca aggtgaaggg gaaacgcagc agtgagcacg 2221 ttcattccca gggccgtgtg atgggggacc gcagtgtgct ctacaagagc ctgaacccca 2281 acctgctggc cgtggtgaca gagagcacag acgcgcacca tgagcgcacc tttattggca 2341 tcttcctcat tgatggcgtc actgggcgta tcattcactc ctctgtgcag aagaaagcca 2401 aaggccctgt ccatatcgtg cattcagaga actgggtggt gtaccagtac tggaacacca 2461 aggctcggcg caacgagttt accgtactgg agctctatga gggcactgag caatacaacg 2521 ccaccgcctt cagctccctg gaccgccccc agctgcccca ggtcctccag cagtcctata 2581 tcttcccgtc ctccatcagt gccatggagg ccaccatcac cgaacggggc atcaccagcc 2641 gacacctgct gattggacta ccttctggag caattctttc ccttcctaag gctttgctgg 2701 aTCCCCGCCG CCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACTTA ATCCCGTATT 2761 CTCCAGATGT ACAGATACAC GCAGAGCGAT TCATCAACTA TAACCAGACA GTTTCTCGAA 2821 TGCGAGGTAT CTACACAGCT CCCTCGGGTC TGGAGTCCAC TTGTTTGGTT GTGGCCTATG 2881 GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGACGTT CTGAAGGATG 2941 ACTATGACTA CGTGTTAATC AGCAGCGTCC TCTTTGGCCT GGTTTTTGCC ACCATGATCA 3001 CTAAGAGACT GGCACAGGTG AAGCTCCTGA ATCGGGCCTG GCGATACCCA ACTTTCTTGT 3061 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3121 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3181 GAAAGGACGA GAGCCTGCCC GTACACACAC TCTAACGCGT TAAGTCgaca atcaacctct 3241 ggattacaaa atttgtgaaa gatt