Construct: ORF TRCN0000478824

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012913.1_s317c1
Derived from:
ccsbBroadEn_02717
DNA Barcode:
GAGCCTGCCCGTACACACACTCTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EMC1 (23065)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478824
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23065 EMC1 ER membrane protein complex... NM_015047.3 100% 100%
2 human 23065 EMC1 ER membrane protein complex... NM_001271427.2 99.8% 99.8% 1025_1026insGCA
3 human 23065 EMC1 ER membrane protein complex... NM_001271428.2 99.8% 99.8% 1308_1309insGCA
4 human 23065 EMC1 ER membrane protein complex... XM_005245787.2 99.6% 99.6% 1431_1439delAGCAATTCA
5 human 23065 EMC1 ER membrane protein complex... XM_005245788.2 99.5% 99.5% 1025_1026insGCA;1428_1436delAGCAATTCA
6 human 23065 EMC1 ER membrane protein complex... NM_001271429.2 97.7% 97.6% 220_221ins66
7 mouse 230866 Emc1 ER membrane protein complex... NM_001039200.2 88.2% 93.3% (many diffs)
8 mouse 230866 Emc1 ER membrane protein complex... NM_146157.4 87.9% 93% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3045
ORF length:
2979
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggctgagtgg gcttctcgtt tctggctttg ggctacgctg ctgattcctg
121cggccgcggt ctacgaagac caagtgggca agtttgattg gagacagcaa tatgttggga
181aggtcaagtt tgcctccttg gaattttccc ctggatccaa gaagttggtt gtagccacag
241agaagaatgt gattgcagca ttaaattccc gaactgggga gatcttgtgg cgccatgttg
301acaagggcac ggcagaaggg gctgtggatg ccatgctgct gcacggacag gatgtgatca
361ctgtgtccaa tggaggccga atcatgcgtt cctgggagac taacatcggg ggcctgaact
421gggagataac cctggacagt ggcagtttcc aggcacttgg gctggttggc ctgcaggagt
481ctgtaaggta catcgcagtc ctgaagaaga ctacacttgc cctccatcac ctctccagtg
541ggcacctcaa gtgggtggaa catctcccag aaagtgacag catccactac cagatggtgt
601attcttacgg ctctggggtg gtgtgggccc tcggagttgt tcccttcagc catgtgaaca
661ttgtcaagtt taatgtggaa gatggagaga ttgttcagca ggttagggtt tcaactccgt
721ggctgcagca cctgtctgga gcctgtggtg tggtggatga ggctgtcctg gtgtgtcctg
781acccgagctc acgttccctc caaactttgg ctctggagac ggaatgggag ttgagacaga
841tcccactgca gtctctcgac ttagaatttg gaagtggatt ccaaccccgg gtcctgccta
901cccagcccaa cccagtggac gcttcccggg cccagttctt cctgcacttg tccccaagcc
961actatgctct gctgcagtac cattatggaa cgctgagttt gcttaaaaac ttcccacaga
1021ctgccctagt gagctttgcc accactgggg agaagacggt ggctgcagtc atggcctgtc
1081ggaatgaagt gcagaaaagt agcagttctg aagatgggtc aatggggagc ttttcggaga
1141agtctagttc aaaggactct ctggcttgct tcaatcagac ctacaccatt aacctatacc
1201tcgtggagac aggtcggcgg ctgctggaca ccacgataac atttagcctg gaacagagcg
1261gcactcggcc tgagcggctg tatatccagg tgttcttgaa gaaggatgac tcagtgggct
1321accgggcttt ggtgcagaca gaggatcatc tgctactttt cctgcagcag ttggcaggga
1381aggtggtgct gtggagccgt gaggagtccc tggcagaagt ggtgtgccta gagatggtgg
1441acctccccct gactggggca caggccgagc tggaaggaga atttggcaaa aaggcagatg
1501gcttgctggg gatgttcctg aaacgcctct cgtctcagct tatcctgctg caagcatgga
1561cttcccacct ctggaaaatg ttttatgatg ctcggaagcc ccggagtcag attaagaatg
1621agatcaacat tgacaccctg gccagagatg aattcaacct ccagaagatg atggtgatgg
1681taacagcctc aggcaagctt tttggcattg agagcagctc tggcaccatc ctgtggaaac
1741agtatctacc caatgtcaag ccagactcct cctttaaact gatggtccag agaactactg
1801ctcatttccc ccatccccca cagtgcaccc tgctggtgaa ggacaaggag tcgggaatga
1861gttctctgta tgtcttcaat cccatttttg ggaagtggag tcaggtagct cccccagtgc
1921tgaagcgccc catcttgcag tccttgcttc tcccagtcat ggatcaagac tacgccaagg
1981tgttgctgtt gatagatgat gaatacaagg tcacagcttt tccagccact cggaatgtct
2041tgcgacagct acatgagctt gccccttcca tcttcttcta tttggtggat gcagagcagg
2101gacggctgtg tggatatcgg cttcgaaagg atctcaccac tgagctgagt tgggagctga
2161ccattccccc agaagtacag cggatcgtca aggtgaaggg gaaacgcagc agtgagcacg
2221ttcattccca gggccgtgtg atgggggacc gcagtgtgct ctacaagagc ctgaacccca
2281acctgctggc cgtggtgaca gagagcacag acgcgcacca tgagcgcacc tttattggca
2341tcttcctcat tgatggcgtc actgggcgta tcattcactc ctctgtgcag aagaaagcca
2401aaggccctgt ccatatcgtg cattcagaga actgggtggt gtaccagtac tggaacacca
2461aggctcggcg caacgagttt accgtactgg agctctatga gggcactgag caatacaacg
2521ccaccgcctt cagctccctg gaccgccccc agctgcccca ggtcctccag cagtcctata
2581tcttcccgtc ctccatcagt gccatggagg ccaccatcac cgaacggggc atcaccagcc
2641gacacctgct gattggacta ccttctggag caattctttc ccttcctaag gctttgctgg
2701aTCCCCGCCG CCCCGAGATC CCAACAGAAC AAAGCAGAGA GGAGAACTTA ATCCCGTATT
2761CTCCAGATGT ACAGATACAC GCAGAGCGAT TCATCAACTA TAACCAGACA GTTTCTCGAA
2821TGCGAGGTAT CTACACAGCT CCCTCGGGTC TGGAGTCCAC TTGTTTGGTT GTGGCCTATG
2881GTTTGGACAT TTACCAAACT CGAGTCTACC CATCCAAGCA GTTTGACGTT CTGAAGGATG
2941ACTATGACTA CGTGTTAATC AGCAGCGTCC TCTTTGGCCT GGTTTTTGCC ACCATGATCA
3001CTAAGAGACT GGCACAGGTG AAGCTCCTGA ATCGGGCCTG GCGATACCCA ACTTTCTTGT
3061ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
3121AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
3181GAAAGGACGA GAGCCTGCCC GTACACACAC TCTAACGCGT TAAGTCgaca atcaacctct
3241ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)