Construct: ORF TRCN0000478832
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001223.1_s317c1
- Derived from:
- ccsbBroadEn_04353
- DNA Barcode:
- AGTAGGTTCAACCGCAAACAGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOA1 (84273)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478832
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84273 | NOA1 | nitric oxide associated 1 | NM_032313.4 | 99.9% | 100% | 432T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2160
- ORF length:
- 2094
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcccgctcgc ctaccgttca ggctgctgag ccttttcctt cgtggatccg 121 ctcccacggc agcgcgccat ggcctccggg agccgctcct ggagaggagg tgcgctgccg 181 cctcctcctt ccagcactca tcgagtctgg gacgcgagct tccttatgac cccgtggaca 241 cggagggctt tggagaaggt ggtgacatgc aggagcgttt tctgttcccg gagtacatcc 301 tggatccgga gccgcaaccc acccgcgaaa agcagctgca ggagctccag caacagcagg 361 aggaggagga gcgacagagg cagcagcggc gggaggagcg gcgacagcaa aacctacggg 421 ccaggtcccg ggagcacccg gtcgtggggc acccggaccc ggcattgccg cccagcggcg 481 tgaactgctc gggctgcggg gcagagctgc actgccagga cgccggagtg cccggctacc 541 tgccccgaga gaagttcctc cgcacggcgg aggcagacgg cgggctggca cggaccgtgt 601 gccagcgctg ctggctgctg tcgcaccacc ggcgcgctct acgcctgcag gtgagccgcg 661 agcagtacct ggagctggtg agcgccgcgt tgcggcggcc cggcccctcc ctggtgctct 721 acatggtgga cctgctggac ctgcccgacg ccctgctgcc cgacttgccc gcgctggtgg 781 gccccaagca gctgatcgtg ctgggaaaca aagtggacct cctgccccag gatgctcctg 841 gctaccggca gaggctgcgg gagcgactgt gggaggactg tgcccgcgcc gggctcctgc 901 tggcccctgg ccaccaaggg ccacagcgcc ccgtcaagga cgagccacag gacggggaga 961 atccgaatcc gccgaactgg tcccgcacag tggtcaggga cgtgcggctg atcagcgcca 1021 agaccggcta tggagtggaa gagttgatct ctgcccttca gcgctcctgg cgctaccgtg 1081 gggacgtcta cttagtgggc gccaccaacg ccggcaaatc cactctcttt aacacgctcc 1141 tggagtccga ttactgcact gccaagggct ccgaggccat cgacagagcc accatctccc 1201 cttggccagg tactacatta aaccttctga agtttcctat ttgcaaccca actccttaca 1261 gaatgtttaa aaggcatcaa agacttaaaa aagattcaac tcaagctgaa gaagatctta 1321 gtgagcaaga acaaaatcag cttaatgtcc tcaaaaagca tggttatgtc gtaggaagag 1381 ttggaaggac attcttgtat tcagaagaac agaaggataa cattcccttt gagtttgatg 1441 ctgattcact tgcctttgac atggaaaatg accctgttat gggtacacac aaatccacca 1501 aacaagtaga attgactgca caagatgtga aagatgccca ctggttttat gacacccctg 1561 gaattacaaa agaaaattgt attttaaatc ttctaacaga aaaagaagta aatattgttt 1621 tgccaacaca gtccattgtt ccaagaactt ttgtgcttaa accaggaatg gttctgtttt 1681 tgggtgctat aggccgcata gatttcctgc agggaaatca gtcagcttgg tttacagtcg 1741 tggcttccaa catcctccct gtgcatatca ccTCCTTGGA CAGGGCAGAC GCTCTGTATC 1801 AGAAGCATGC AGGTCATACG TTACTCCAGA TTCCAATGGG TGGAAAAGAA CGAATGGCAG 1861 GATTTCCTCC TCTTGTTGCT GAAGACATTA TGTTAAAAGA AGGACTGGGG GCATCTGAAG 1921 CAGTGGCCGA CATCAAGTTT TCCTCTGCAG GTTGGGTTTC AGTAACACCT AATTTTAAGG 1981 ACAGACTGCA TCTCCGAGGC TATACACCTG AAGGAACAGT TTTGACCGTC CGGCCCCCTC 2041 TCTTGCCATA TATTGTTAAC ATCAAAGGAC AGCGCATCAA GAAAAGTGTG GCCTATAAAA 2101 CCAAGAAGCC TCCTTCCCTT ATGTACAACG TGAGGAAGAA GAAAGGAAAG ATAAATGTAT 2161 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2221 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2281 TTTATATATC TTGTGGAAAG GACGAAGTAG GTTCAACCGC AAACAGATCA CGCGTTAAGT 2341 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt