Construct: ORF TRCN0000478842
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005065.1_s317c1
- Derived from:
- ccsbBroadEn_03444
- DNA Barcode:
- ATGCGAACAAGCGTTACTGCAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAF1 (54623)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478842
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54623 | PAF1 | PAF1 homolog, Paf1/RNA poly... | NM_019088.4 | 100% | 100% | |
| 2 | human | 54623 | PAF1 | PAF1 homolog, Paf1/RNA poly... | NM_001256826.2 | 82.6% | 64.4% | 45_46ins30;1150_1151ins184;1380_1455del |
| 3 | human | 54623 | PAF1 | PAF1 homolog, Paf1/RNA poly... | NR_046384.2 | 64.8% | (many diffs) | |
| 4 | mouse | 54624 | Paf1 | Paf1, RNA polymerase II com... | NM_019458.3 | 89.7% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1659
- ORF length:
- 1593
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcccaccatc cagacccagg cccagcggga ggatggccac aggcccaatt 121 cccaccggac tctgcctgag aggtctggag tggtctgccg agtcaagtac tgcaatagcc 181 tccctgatat ccccttcgac cccaagttca tcacctaccc cttcgaccag aacaggttcg 241 tccagtacaa agccacttcc ttggagaaac agcacaaaca tgacctcctg actgagccag 301 acctgggggt caccatcgat ctcatcaatc ctgacaccta ccgcatcgac cccaatgttc 361 ttctagatcc agctgatgag aaacttttgg aagaggagat tcaggccccc accagctcca 421 agagatccca gcagcacgcg aaggtggtgc catggatgcg aaagacagag tacatctcca 481 ctgagttcaa ccgttatggc atctccaatg agaagcctga ggtcaagatt ggggtttctg 541 tgaagcagca gtttaccgag gaagaaatat acaaagacag ggatagccag atcacagcca 601 ttgagaagac ttttgaggat gcccagaaat caatctcaca gcattacagc aaaccccgag 661 tcacaccggt ggaggtcatg cctgtcttcc cagactttaa gatgtggatc aatccatgtg 721 ctcaggtgat ctttgactca gacccagccc ccaaggacac gagtggtgca gctgcgttgg 781 agatgatgtc tcaggccatg attaggggca tgatggatga ggaagggaac cagtttgtgg 841 cctatttcct gcctgtagaa gagacgttga agaaacgaaa gcgggaccag gaggaggaga 901 tggactatgc accagatgat gtgtatgact acaaaattgc tcgggagtac aactggaacg 961 tgaagaacaa agctagcaag ggctatgagg aaaactactt cttcatcttc cgagagggtg 1021 acggggttta ctacaatgag ttggaaacca gggtccgcct tagtaagcgc cgggccaagg 1081 ctggggttca gtcaggcacc aacgccctgc ttgtggtcaa acatcgggac atgaatgaga 1141 aggaactgga agctcaggag gcacggAAGG CCCAGCTAGA AAACCACGAA CCGGAGGAGG 1201 AAGAGGAAGA GGAGATGGAG ACAGAAGAGA AAGAAGCTGG GGGCTCAGAT GAGGAGCAGG 1261 AGAAGGGCAG CAGCAGTGAG AAGGAGGGCA GTGAAGATGA GCACTCGGGC AGCGAGAGTG 1321 AACGGGAGGA AGGTGACAGG GACGAGGCCA GTGACAAGAG TGGCAGTGGT GAGGACGAGA 1381 GCAGCGAGGA TGAGGCCCGG GCTGCCCGTG ACAAAGAGGA GATCTTTGGC AGTGATGCTG 1441 ATTCTGAGGA CGATGCCGAC TCTGATGATG AGGACAGAGG ACAGGCCCAA GGTGGCAGTG 1501 ACAATGATTC AGACAGCGGC AGCAATGGGG GTGGCCAGCG GAGCCGGAGC CACAGCCGCA 1561 GCGCCAGTCC CTTCCCCAGT GGCAGCGAGC ACTCGGCCCA GGAGGATGGC AGTGAAGCTG 1621 CAGCTTCTGA TTCCAGTGAA GCTGATAGTG ACAGTGACTG CCCAACTTTC TTGTACAAAG 1681 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1741 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1801 ACGAATGCGA ACAAGCGTTA CTGCAGGAAC GCGTTAAGTC gacaatcaac ctctggatta 1861 caaaatttgt gaaagatt