Transcript: Human NM_019088.4

Homo sapiens PAF1 homolog, Paf1/RNA polymerase II complex component (PAF1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-10
Taxon:
Homo sapiens (human)
Gene:
PAF1 (54623)
Length:
2200
CDS:
243..1838

Additional Resources:

NCBI RefSeq record:
NM_019088.4
NBCI Gene record:
PAF1 (54623)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_019088.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005455 GCCGAGTCAAGTACTGCAATA pLKO.1 334 CDS 100% 10.800 15.120 N PAF1 n/a
2 TRCN0000342730 GCCGAGTCAAGTACTGCAATA pLKO_005 334 CDS 100% 10.800 15.120 N PAF1 n/a
3 TRCN0000005454 CGTGAAGAACAAAGCTAGCAA pLKO.1 1136 CDS 100% 3.000 4.200 N PAF1 n/a
4 TRCN0000010939 GATGCGAAAGACAGAGTACAT pLKO.1 632 CDS 100% 4.950 3.960 N PAF1 n/a
5 TRCN0000342666 GATGCGAAAGACAGAGTACAT pLKO_005 632 CDS 100% 4.950 3.960 N PAF1 n/a
6 TRCN0000005453 GCTGGTTCAGACACCATTATT pLKO.1 1856 3UTR 100% 15.000 10.500 N PAF1 n/a
7 TRCN0000342733 GCTGGTTCAGACACCATTATT pLKO_005 1856 3UTR 100% 15.000 10.500 N PAF1 n/a
8 TRCN0000198521 GAGATGGACTATGCACCAGAT pLKO.1 1074 CDS 100% 4.050 2.835 N Paf1 n/a
9 TRCN0000005456 GCAGTGACAATGATTCAGACA pLKO.1 1672 CDS 100% 2.640 1.848 N PAF1 n/a
10 TRCN0000342732 GCAGTGACAATGATTCAGACA pLKO_005 1672 CDS 100% 2.640 1.848 N PAF1 n/a
11 TRCN0000087583 AGGAGGAAGAGGAAGAGGAAA pLKO.1 1372 CDS 100% 4.950 2.475 Y Adam32 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019088.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03444 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03444 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478842 ATGCGAACAAGCGTTACTGCAGGA pLX_317 31.1% 100% 100% V5 n/a
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