Construct: ORF TRCN0000478845
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002988.1_s317c1
- Derived from:
- ccsbBroadEn_08095
- DNA Barcode:
- TAGCACCCCTCGGCTAGGTAGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC27A6 (28965)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478845
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28965 | SLC27A6 | solute carrier family 27 me... | NM_001017372.3 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 28965 | SLC27A6 | solute carrier family 27 me... | NM_001317984.1 | 99.7% | 99.5% | (many diffs) |
| 3 | human | 28965 | SLC27A6 | solute carrier family 27 me... | NM_014031.4 | 99.7% | 99.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1923
- ORF length:
- 1857
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tctgtcatgg ctaacagttc taggggctgg aatggtcgtc ctgcacttcg 121 tgcagaaact cctgttcact tacttttggg atgacttctg gttcgtgttg aaggtggtgc 181 tcattataat tcggctgaag aagtacgaaa agagagggga gctggtgact gtgctggata 241 aattcttgag tcatgccaaa agacaacctc ggaaaccttt catcatctat gagggagaca 301 tctacaccta tcaggatgta gacaaaagga gcagcagagt ggcccatgtc ttcctgaacc 361 attcctctct gaaaaagggg gacacggtgg ctctgctgat gagcaatgag ccggacttcg 421 ttcacgtgtg gttcggcctc gccaagctgg gctgcgtggt ggcctttctc aacaccaaca 481 ttcgctccaa ctccctcctg aattgcatcc gcgcctgtgg gcccagagcc ctagtggtgg 541 gcgcagattt gcttgggacg gtagaagaaa tccttccaag cctctcagaa aatatcagtg 601 tttgggggat gaaagattct gttccacaag gtgtaatttc actcaaagaa aaactgagca 661 cctcacctga tgagcccgtg ccacgcagcc accatgttgt ctcactcatc aagtctactt 721 gtctttacat ttttacctct ggaacaacag gtctaccaaa agcagctgtg attagtcagc 781 tgcaggtttt aaggggttct gctgtcctgt gggcttttgg ttgtactgct catgacattg 841 tttatataac ccttcctctg tatcatagtt cagcagctat cctgggaatt tctggatgtg 901 ttgagttggg tgccacttgt gtgttaaaga agaaattttc agcaagccag ttttggagtg 961 actgcaagaa gtatgatgtg actgtgtttc agtatattgg agaactttgt cgctaccttt 1021 gcaaacaatc taagagagaa ggagaaaagg atcataaggt gcgtttggca attggaaatg 1081 gcatacggag tgatgtatgg agagaatttt tagacagatt tggaaatata aaggtgtgtg 1141 aactttatgc agctaccgaa tcaagcatat ctttcatgaa ctacactggg agaattggag 1201 caattgggag aacaaatttg ttttacaaac ttctttccac ttttgactta ataaagtatg 1261 actttcagaa agatgaaccc atgagaaatg agcagggttg gtgtattcat gtgaaaaaag 1321 gagaacctgg acttctcatt tctcgagtga atgcaaaaaa tcccttcttt ggctatgctg 1381 ggccttataa gcacacaaaa gacaaattgc tttgtgatgt ttttaagaag ggagatgttt 1441 accttaatac tggagactta atagtccagg atcaggacaa tttcctttat ttttgggacc 1501 gtactggaga cactttcaga tggaaaggag aaaatgtcgc aaccaCTGAG GTTGCTGATG 1561 TTATTGGAAT GTTGGATTTC ATACAGGAAG CAAACGTCTA TGGTGTGGCT ATATCAGGTT 1621 ATGAAGGAAG AGCAGGAATG GCTTCTATTA TTTTAAAACC AAATACATCT TTAGATTTGG 1681 AAAAAGTTTA TGAACAAGTT GTAACATTTC TACCAGCTTA TGCTTGTCCA CGATTTTTAA 1741 GAATTCAGGA AAAAATGGAA GCAACAGGAA CATTCAAACT ATTGAAGCAT CAGTTGGTGG 1801 AAGATGGATT TAATCCACTG AAAATTTCTG AACCACTTTA CTTCATGGAT AACTTGAAAA 1861 AGTCTTATGT TCTACTGACC AGGGAACTTT ATGATCAAAT AATGTTAGGG GAAATAAAAC 1921 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1981 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2041 GGCTTTATAT ATCTTGTGGA AAGGACGATA GCACCCCTCG GCTAGGTAGC GCACGCGTTA 2101 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt