Construct: ORF TRCN0000478876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001959.1_s317c1
- Derived from:
- ccsbBroadEn_11538
- DNA Barcode:
- CCCCTCAGGCCAAATATTATCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNB5 (10681)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478876
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10681 | GNB5 | G protein subunit beta 5 | NM_006578.4 | 33.9% | 27.3% | (many diffs) |
| 2 | human | 10681 | GNB5 | G protein subunit beta 5 | NM_016194.4 | 30.3% | 24.5% | (many diffs) |
| 3 | human | 10681 | GNB5 | G protein subunit beta 5 | XM_011521163.3 | 19.3% | 13.1% | (many diffs) |
| 4 | human | 10681 | GNB5 | G protein subunit beta 5 | XR_001751060.2 | 15.7% | (many diffs) | |
| 5 | mouse | 14697 | Gnb5 | guanine nucleotide binding ... | NM_138719.5 | 31.6% | 27.1% | (many diffs) |
| 6 | mouse | 14697 | Gnb5 | guanine nucleotide binding ... | NM_010313.2 | 28.2% | 24.3% | (many diffs) |
| 7 | mouse | 14697 | Gnb5 | guanine nucleotide binding ... | NR_073186.1 | 13.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 444
- ORF length:
- 378
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aaccgagggg ctgcacgaga acgagacgct GGCGTCGCTG AAGAGCGAGG 121 CCGAGAGCCT CAAGGGCAAG CTGGAGGAGG AGCGAGCCAA GCTGCACGAT GTGGAGCTGC 181 ACCAGGTGGC GGAGCGGGTG GAGGCCCTGG GGCAGTTTGT CATGAAGACC AGAAGGACCC 241 TCAAAGGCCA CGGGAACAAA GTCCTGTGCA TGGACTGGTG CAAAGATAAG AGGAGGATCG 301 TGAGCTCGTC ACAGGATGGG AAGGTGATCG TGTGGGATTC CTTCACCACA AACAAGGTGA 361 GGCATTGTTC CCAGCCACGT TATAGAGGCT TCAGAATCCC AGCCTACTTA AAGGTGCTCT 421 GGTCCAGCTG CCCGGCCCTG TCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 481 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 541 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CCCTCAGGCC 601 AAATATTATC TAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 661 att