Transcript: Human NM_006578.4

Homo sapiens G protein subunit beta 5 (GNB5), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
GNB5 (10681)
Length:
8765
CDS:
47..1108

Additional Resources:

NCBI RefSeq record:
NM_006578.4
NBCI Gene record:
GNB5 (10681)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006578.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000433362 AGCGTCTCCAATATGACTATT pLKO_005 1243 3UTR 100% 13.200 18.480 N GNB5 n/a
2 TRCN0000036890 GCGTTAGCACTCTACGAGTTT pLKO.1 1026 CDS 100% 4.950 6.930 N GNB5 n/a
3 TRCN0000435905 TCCAAAGAAAGCATCATATTT pLKO_005 878 CDS 100% 15.000 10.500 N GNB5 n/a
4 TRCN0000036892 CATGGACTGGTGCAAAGATAA pLKO.1 250 CDS 100% 13.200 9.240 N GNB5 n/a
5 TRCN0000036891 CTGGATACAATGATTACACTA pLKO.1 945 CDS 100% 4.950 3.465 N GNB5 n/a
6 TRCN0000036893 CCTCAGAAACTGGAAACACCT pLKO.1 660 CDS 100% 2.640 1.848 N GNB5 n/a
7 TRCN0000036889 GCTCTGGATCATGGGATCATA pLKO.1 1068 CDS 100% 5.625 3.375 N GNB5 n/a
8 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 6655 3UTR 100% 4.950 2.475 Y ERAP2 n/a
9 TRCN0000127601 CGCTTGTAATCCCAGCACTTT pLKO.1 1993 3UTR 100% 4.950 2.475 Y CCDC30 n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 6656 3UTR 100% 13.200 6.600 Y LIAS n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 5236 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 5236 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006578.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02505 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02505 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000471530 ATTAATACCCAGACTTTTCGGCAT pLX_317 36% 100% 100% V5 n/a
4 ccsbBroadEn_11538 pDONR223 100% 33.9% 27.3% None (many diffs) n/a
5 ccsbBroad304_11538 pLX_304 0% 33.9% 27.3% V5 (many diffs) n/a
6 TRCN0000478876 CCCCTCAGGCCAAATATTATCTAA pLX_317 88.9% 33.9% 27.3% V5 (many diffs) n/a
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