Construct: ORF TRCN0000478878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016885.1_s317c1
- Derived from:
- ccsbBroadEn_05289
- DNA Barcode:
- GCCCCCCGATTTAGATCTATGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERHL2 (253190)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478878
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253190 | SERHL2 | serine hydrolase like 2 | NM_014509.5 | 100% | 100% | |
| 2 | human | 253190 | SERHL2 | serine hydrolase like 2 | XM_017028739.2 | 95.5% | 95.5% | 285_286ins42 |
| 3 | human | 253190 | SERHL2 | serine hydrolase like 2 | XM_024452196.1 | 92% | 92% | 613_693del |
| 4 | human | 253190 | SERHL2 | serine hydrolase like 2 | XR_001755198.2 | 81.1% | 1_23del;635_636ins35;806_807ins124 | |
| 5 | human | 253190 | SERHL2 | serine hydrolase like 2 | NM_001284334.2 | 79.6% | 79.6% | 20_21ins150;135_136ins42 |
| 6 | human | 253190 | SERHL2 | serine hydrolase like 2 | XM_024452197.1 | 67.3% | 67.4% | 285_286ins42;571_651del;771_771delGins211 |
| 7 | human | 253190 | SERHL2 | serine hydrolase like 2 | XR_244363.3 | 58% | 1_153del;685_686ins115;981_1310del | |
| 8 | human | 94009 | SERHL | serine hydrolase like (pseu... | NR_027786.1 | 55.5% | (many diffs) | |
| 9 | human | 253190 | SERHL2 | serine hydrolase like 2 | NR_104301.2 | 44.5% | (many diffs) | |
| 10 | human | 253190 | SERHL2 | serine hydrolase like 2 | NR_104300.2 | 44.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1011
- ORF length:
- 942
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgagaac gccgcaccag gtctgatctc agagctgaag ctggctgtgc 121 cctggggcca catcgcagcc aaagcctggg gctccctgca gggccctcca gttctctgcc 181 tgcacggctg gctggacaat gccagctcct tcgacagact catccctctt ctcccgcaag 241 acttttatta cgttgccatg gatttcggag gtcatgggct ctcgtcccat tacagcccag 301 gtgtcccata ttacctccag acttttgtga gtgagatccg aagagttgtg gcagccttga 361 aatggaatcg attctccatt ctgggccaca gcttcggtgg cgtcgtgggc ggaatgtttt 421 tctgtacctt ccccgagatg gtggataaac ttatcttgct ggacacgccg ctctttctcc 481 tggaatcaga tgaaatggag aacttgctga cctacaagcg gagagccata gagcacgtgc 541 tgcaggtaga ggcctcccag gagccctcgc acgtgttcag cctgaagcag ctgctgcaga 601 ggttactgaa gagcaatagc cacttGAGTG AGGAGTGCGG GGAGCTTCTC CTGCAAAGAG 661 GAACCACGAA GGTGGCCACA GGTCTGGTTC TGAACAGAGA CCAGAGGCTC GCCTGGGCAG 721 AGAACAGCAT TGACTTCATC AGCAGGGAGC TGTGTGCGCA TTCCATCAGG AAGCTGCAGG 781 CCCATGTCCT GTTGATCAAA GCAGTCCACG GATATTTTGA TTCAAGACAG AATTACTCTG 841 AGAAGGAGTC CCTGTCGTTC ATGATAGACA CGATGAAATC CACCCTCAAA GAGCAGTTCC 901 AGTTTGTGGA AGTCCCAGGC AATCACTGTG TCCACATGAG CGAACCCCAG CACGTGGCCA 961 GTATCATCAG CTCCTTCTTA CAGTGCACAC ACATGCTCCC AGCCCAGCTG TTGCCAACTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGAGCCC CCCGATTTAG ATCTATGTTC ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt