Construct: ORF TRCN0000478880
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014722.2_s317c1
- Derived from:
- ccsbBroadEn_11780
- DNA Barcode:
- CCAGACGGCGAAGTGCTCAGCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC28A (25901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478880
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25901 | CCDC28A | coiled-coil domain containi... | NM_015439.2 | 47.4% | 47.4% | 1_432del |
| 2 | human | 25901 | CCDC28A | coiled-coil domain containi... | XM_011535728.3 | 19.5% | 19.7% | 1_432del;594_594delTins229 |
| 3 | mouse | 215814 | Ccdc28a | coiled-coil domain containi... | NM_144820.3 | 60.5% | 67.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agaaaagacc aaacctcagg gtggagaggg caaaggcgct cagtcaacTC 121 CGATCCAGCA CTCCTTCCTC ACTGATGTCT CAGATGTTCA GGAGATGGAG AGAGGGCTGC 181 TCAGTCTTTT GAATGATTTC CACTCTGGAA AACTTCAAGC ATTTGGAAAT GAATGTTCCA 241 TTGAACAGAT GGAACATGTT CGGGGAATGC AGGAGAAATT AGCTCGCTTG AATTTGGAGC 301 TCTATGGGGA GTTAGAGGAA CTTCCTGAGG ATAAGAGAAA AACAGCCAGT GACTCCAATC 361 TGGATAGGCT TCTGTCAGAT TTAGAAGAAT TGAATTCTTC CATACAAAAA CTCCATTTGG 421 CAGATGCACA AGATGTTCCA AATACTTCTG CTAGCTACCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 ACCAGACGGC GAAGTGCTCA GCGTAACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt