Construct: ORF TRCN0000478889
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006210.1_s317c1
- Derived from:
- ccsbBroadEn_05438
- DNA Barcode:
- CCAATGTACGGTTCGAATCTACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R60 (338398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478889
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338398 | TAS2R60 | taste 2 receptor member 60 | NM_177437.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1023
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatggagac cacatggttc taggatcttc ggtgactgac aagaaggcca 121 tcatcttggt taccatttta ctccttttac gcctggtagc aatagcaggc aatggcttca 181 tcactgctgc tctgggcgtg gagtgggtgc tacggagaat gttgttgcct tgtgataagt 241 tattggttag cctaggggcc tctcgcttct gtctgcagtc agtggtaatg ggtaagacca 301 tttatgtttt cttgcatccg atggccttcc catacaaccc tgtactgcag tttctagctt 361 tccagtggga cttcctgaat gctgccacct tatggtcctc tacctggctc agtgtcttct 421 attgtgtgaa aattgctacc ttcacccacc ctgtcttctt ctggctaaag cacaagttgt 481 ctgggtggct accatggatg ctcttcagct ctgtagggct ctccagcttc accaccattc 541 tatttttcat aggcaaccac agaatgtatc agaactattt aaggaaccat ctacaacctt 601 GGAATGTCAC TGGCGATAGC ATACGGAGCT ACTGTGAGAA ATTCTATCTC TTCCCTCTAA 661 AAATGATTAC TTGGACAATG CCCACTGCTG TCTTTTTCAT TTGCATGATT TTGCTCATCA 721 CATCTCTGGG AAGACACAGG AAGAAGGCTC TCCTTACAAC CTCAGGATTC CGAGAGCCCA 781 GTGTGCAGGC ACACATAAAG GCTCTGCTGG CTCTCCTCTC TTTTGCCATG CTCTTCATCT 841 CATATTTCCT GTCACTGGTG TTCAGTGCTG CAGGTATTTT TCCACCTCTG GACTTTAAAT 901 TCTGGGTGTG GGAGTCAGTG ATTTATCTGT GTGCAGCAGT TCACCCCATC ATTCTGCTCT 961 TCAGCAACTG CAGGCTGAGA GCTGTGCTGA AGAGTCGTCG TTCCTCAAGG TGTGGGACAC 1021 CTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1081 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1141 GGCTTTATAT ATCTTGTGGA AAGGACGACC AATGTACGGT TCGAATCTAC CTACGCGTTA 1201 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt