Construct: ORF TRCN0000478916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012278.1_s317c1
- Derived from:
- ccsbBroadEn_00583
- DNA Barcode:
- TAAATCAAGTTTAACTCAACAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FMO5 (2330)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478916
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2330 | FMO5 | flavin containing dimethyla... | NM_001144830.2 | 100% | 100% | |
| 2 | human | 2330 | FMO5 | flavin containing dimethyla... | XM_006711245.4 | 100% | 100% | |
| 3 | human | 2330 | FMO5 | flavin containing dimethyla... | XM_006711244.4 | 85% | 82.9% | (many diffs) |
| 4 | human | 2330 | FMO5 | flavin containing dimethyla... | NM_001144829.2 | 60.7% | 59.6% | (many diffs) |
| 5 | human | 2330 | FMO5 | flavin containing dimethyla... | NM_001461.4 | 52.8% | 51.9% | (many diffs) |
| 6 | human | 2330 | FMO5 | flavin containing dimethyla... | XM_005272946.5 | 52.8% | 51.9% | (many diffs) |
| 7 | human | 2330 | FMO5 | flavin containing dimethyla... | XM_005272947.5 | 52.8% | 51.9% | (many diffs) |
| 8 | human | 2330 | FMO5 | flavin containing dimethyla... | XM_005272948.5 | 52.8% | 51.9% | (many diffs) |
| 9 | human | 2330 | FMO5 | flavin containing dimethyla... | XM_011509350.3 | 52.8% | 51.9% | (many diffs) |
| 10 | human | 2330 | FMO5 | flavin containing dimethyla... | XM_011509351.3 | 41% | 40.2% | (many diffs) |
| 11 | human | 2330 | FMO5 | flavin containing dimethyla... | XM_017000801.2 | 41% | 40.2% | (many diffs) |
| 12 | human | 2330 | FMO5 | flavin containing dimethyla... | XM_017000802.2 | 41% | 40.2% | (many diffs) |
| 13 | mouse | 14263 | Fmo5 | flavin containing monooxyge... | XM_006500996.2 | 34.4% | 33.1% | (many diffs) |
| 14 | mouse | 14263 | Fmo5 | flavin containing monooxyge... | XM_017319455.1 | 34.4% | 33.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 921
- ORF length:
- 855
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac taagaaaaga attgctgtga ttgggggagg agtgagcggg ctctcttcca 121 tcaagtgctg cgtagaagaa ggcttggaac ctgtctgctt tgaaaggact gatgacatcg 181 gagggctctg gaggttccag gaaaatcctg aagaaggaag ggccagtatt tacaaatcag 241 tgatcatcaa tacttctaaa gagatgatgt gcttcagtga ctatccaatc ccagatcatt 301 atcccaactt catgcataat gcccaggtcc tggagtattt caggatgtat gccaaagaat 361 ttgaccttct aaagtatatt cgatttaaga ccactgtgtg cagtgtgaag aagcagcctg 421 attttgccac ttcaggccaa tgggaagtgg tcactgaatc tgaagggaaa aaggagatga 481 atgtctttga tggagtcatg gtttgcactg gccatcacac caatgctcat ctacctctgg 541 aaagcttccc tGGAATTGAG AAGTTCAAAG GGCAGTACTT CCACAGTCGA GACTATAAGA 601 ACCCAGAGGG ATTCACTGGA AAGAGAGTCA TTATAATTGG CATTGGGAAT TCTGGAGGGG 661 ATCTGGCTGT AGAGATTAGC CAAACAGCCA AGCAGGTTTT CCTCAGCACC AGGAGAGGGG 721 CTTGGATCCT GAATCGTGTA GGGGACTACG GATATCCTGC TGATGTGTTG TTCTCTTCTC 781 GACTTACACA TTTTATATGG AAGATCTGTG GCCAATCATT AGCAAACAAA TATTTGGAAA 841 AAAAGATAAA CCAAAGGTTT GACCATGAAA TGTTTGGCCT GAAGCCTAAA CACAGGTCTA 901 AAGACATTGC CCTCACAGAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 961 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1021 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATAAA TCAAGTTTAA 1081 CTCAACAAAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt