Construct: ORF TRCN0000478928
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011412.1_s317c1
- Derived from:
- ccsbBroadEn_02158
- DNA Barcode:
- TACTGTGACCAATTTTTATACCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM189A2 (9413)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478928
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9413 | FAM189A2 | family with sequence simila... | NM_001127608.2 | 100% | 100% | |
| 2 | human | 9413 | FAM189A2 | family with sequence simila... | NM_004816.4 | 100% | 100% | |
| 3 | human | 9413 | FAM189A2 | family with sequence simila... | XM_024447718.1 | 100% | 100% | |
| 4 | human | 9413 | FAM189A2 | family with sequence simila... | XM_017015324.1 | 94.1% | 94% | 1_77delinsATGATA;83delC |
| 5 | human | 9413 | FAM189A2 | family with sequence simila... | XM_017015325.1 | 74.8% | 61.8% | 854_855ins293;1011_1012ins46 |
| 6 | human | 9413 | FAM189A2 | family with sequence simila... | NM_001347995.1 | 74.6% | 74.6% | 1_459del |
| 7 | human | 9413 | FAM189A2 | family with sequence simila... | XR_001746421.1 | 42.2% | (many diffs) | |
| 8 | mouse | 381217 | Fam189a2 | family with sequence simila... | XM_006527156.3 | 65.2% | 67.4% | (many diffs) |
| 9 | mouse | 381217 | Fam189a2 | family with sequence simila... | NM_001114174.1 | 63.4% | 65.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1419
- ORF length:
- 1350
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatactcctg gtaaacctct ttgtgctgct ctctgtggtt tgtgtcctct 121 taaatctagc tggatttatc ctaggctgcc aaggggccca gtttgtgtcc agcgtgccca 181 ggtgtgatct ggtggactta ggtgaaggca agatttgctt ctgttgtgaa gaatttcaac 241 cagccaagtg cacagacaaa gaaaatgcct tgaaactctt tccggttcag ccctgtagtg 301 ctgttcacct tctacttaag aaagtcctct ttgccctgtg tgccttgaat gccctgacca 361 ccaccgtctg cttggtggcc gctgccctcc gctacctcca gatattcgca accaggagat 421 cctgcatcga tgaatcccag atttctgctg aagaagcgga ggatcatgga cgcatccccg 481 accctgatga ttttgtgccg cctgtgcctc ccccttccta ttttgccacg ttttactcgt 541 gcacaccccg gatgaaccgc aggatggttg gtcctgatgt tattcccctg ccacacatct 601 acggagctcg aatcaaaggt gtggaagtgt tctgtcctct ggatcccccg ccgccatatg 661 aagctgtggt gagccagatg gaccaggagc agggatcttc attccaaatg tcagaaggat 721 cagaagctgc tgtgatccca ttggatctgg gctgcacaca agtgactcaa gatggggaca 781 ttcctaacat acctgccgaa gaaaatgcat ccacctcaac tcccagttca accctggtgc 841 gtcctatcag aagccggaga gccctcccac ccttgaggac caggtcgaag agtgaccctg 901 tgctccatcc ttctgaggag agagctgccc cagtgctcag ctgtgaagct gcaacacaga 961 ctgaaaggag actggatctg gctgcagtga cTCTGAGGAG AGGCTTGAGA TCTAGAGCTT 1021 CGCGATGCAG ACCGCGGTCT TTGATAGATT ACAAATCCTA CATGGACACC AAGCTGCTGG 1081 TGGCGAGGTT CCTGGAGCAG TCCTCTTGTA CCATGACCCC AGACATCCAT GAACTTGTAG 1141 AAAACATTAA ATCTGTTTTG AAATCTGATG AGGAGCACAT GGAGGAAGCC ATCACAAGTG 1201 CCAGTTTTCT AGAACAGATA ATGGCCCCAT TGCAGCCCAG CACATCCAGG GCCCACAAGC 1261 TGCCCTCGCG GAGACAGCCT GGCCTGCTGC ACCTCCAGAG CTGCGGCGAC CTTCACACCT 1321 TCACACCAGC GGGGAGGCCC CGAGCCGAGA GGAGGCCCCG GCGAGTGGAG GCTGAGCGGC 1381 CACACAGCCT CATTGGGGTC ATCCGAGAGA CTGTCCTGTT GCCAACTTTC TTGTACAAAG 1441 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1501 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1561 ACGATACTGT GACCAATTTT TATACCCAAC GCGTTAAGTC gacaatcaac ctctggatta 1621 caaaatttgt gaaagatt